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Microarrays are becoming the platform of choice for the analysis of complex genomes, transcriptomes and proteomes. For a number of applications, however, sample or analyte abundance constraints limit the usefulness of microarrays. Rolling circle amplification (RCA) has previously been shown to be a signal amplification method that is useful in these applications on glass microarrays. This report describes...
Conventional DNA hybridization assay kinetics depends solely on the diffusion of target to surface-bound probes, causing long hybridization times. In this study, we examined the possibilities of accelerating the hybridization process by using microfluidic channels (“biochannels”) made of polycarbonate, optionally with an integrated pump. We produced two different devices to study these effects: first,...
Plasmids pLTV1 and pHV33, capable of replicating in both Gram + and Gram - bacterial hosts (shuttle vectors), when derived from the Escherichia coli strain HB101, were inactive in an electro-transformation assay employing theBacillus anthracis strains ΔAmes-1 and ΔV1B-1 as recipients. The same plasmids isolated from the DNA methyltransferase (MTase)-deficient E. coli strain GM2929...
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