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In countries where highly pathogenic avian influenza virus (HPAIV) H5N1 is endemic and controlled by vaccination, post-vaccination serological monitoring is essential to differentiate vaccinated poultry from those that are infected. The objectives of this study were to validate two experimental ELISAs that detect antibodies raised against the M2e protein of avian influenza virus that can be used for...
Leptospirosis is an endemic zoonotic disease in tropical countries. However, detection using the microagglutination test (MAT) as a gold standard is difficult to perform in Indonesia. Therefore, recombinant LipL32 protein (rLipL32) has been studied as an antigen for an ELISA test to detect Leptospirosis. We produced rLipL32 using the pRSET-C vector and expressed it in E. coli BL21 (DE3) competent...
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