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Objective: To study the effects of GM-CSF and IL-1β, both implicated in tissue damage in arthritis, on articular chondrocyte proliferation and metabolism, and to explore their agonist/antagonist effects. Methods: Chondrocytes were obtained from 1-month-old rats. First-passage monolayers were incubated for 24h with or without GM-CSF and/or IL-1β, and labeled with 3 H-thymidine, 35 S–SO...
Objectifs. – Mieux caractériser les facteurs sécrétés in vitro par les membranes synoviales rhumatoïdes et arthrosiques inhibant la synthèse d'ADN par les chondrocytes articulaires humains en culture, et étendre ces données au liquide synovial.Matériel et méthodes. – Les tissus synoviaux, les liquides synoviaux et les cartilages articulaires étaient obtenus après opération chirurgicale de deux patients...
Aims.– To further characterize factors secreted in vitro by osteoarthritic and rheumatoid arthritis synovial membranes that inhibit DNA synthesis by cultured human articular chondrocytes, and extend these findings to synovial fluid.Material and methods.– Synovial tissue, synovial fluid and articular cartilage were obtained at surgery from two patients suffering rheumatoid arthritis and two other patients...
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