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The α 1 -adrenoceptor subtype mediating noradrenaline (NA)-induced contractions of rat epididymal vas deferens in Ca 2+ -free/EGTA (1 mM) medium was studied using competitive antagonists. The effects of chloroethylclonidine (CEC) was investigated in Ca 2+ -free and normal Krebs' medium and RT-PCR was used to identify α 1 -adrenoceptor specific mRNA in epididymal...
The effects of agents that modulate intracellular release of calcium and protein kinase C (PKC) activation on noradrenaline (NA)-induced contractions of epididymal vas deferens in calcium-free/EGTA (1 mM) medium were investigated. NA (100 μM) or methoxamine (100 μM) evoked repeatable contractions. Clonidine (100 - 300 μM) was ineffective. The contractions to NA were reduced by procaine (1 - 10...
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