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Our previous patch-clamp studies showed that depolarization activates a K + -specific current in the plasma membrane of the budding yeast, Saccharomyces cerevisiae [Gustin et al. (1986) Science 233, 1195-1197]. The Yeast Genome Sequencing Project has now uncovered on the left arm of chromosome X an open reading frame (ORF) that predicts a 77-kDa protein reminiscent of a shaker-like α subunit...