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The current study compared the applicability of protocols to extract DNA from formalin fixed heart tissues that have been preserved for more than 50years. Ten methods were tested: a cetyltrimethylammonium bromide (CTAB) standard protocol, seven variants of this standard protocol, and two commercial kits. In the case of younger specimens (fixed in 1951, 1934, or 1914), extracts with DNA concentrations⩾10...