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Two enzyme assays using either radiolabelled or non-labelled substrates were applied to detect dihydroflavonol 4-reductase (DFR) activity in lignocellulosic tissues. The successful detection of DFR in these tissues rich in polyphenols relied on the addition of sufficient amounts of polyethylene glycol. DFR activity is measured in sapwood and inner bark of different conifer species.
Phenolic extractives have been isolated from Douglas-fir sapwood and heartwood and identified by NMR and mass spectroscopy. Pinocembrin, [5,5 ]-bisdihydroquercetin and two lignans, pinoresinol and 2,3-dihydro-2-(4 -O-β-glucopyranosyl-3 -methoxyphenyl)-3-hydroxymethyl-5- (3-hydroxypropyl)-7-methoxy-benzofuran are reported for the first time in these tissues. The chemical structures of these phenolic...
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