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The recombinant raw starch‐degrading enzyme (LsA175) which harbors the lsa175 gene from Laceyella sacchari LP175 is optimized for expression in Escherichia coli BL21 (DE3). The recombinant strain provides an alternative avenue for enzyme production in a stirrer fermenter with tolerance to shear force and avoids losing stability by protease existing in the native strain. The maximum enzyme production...
The thermotolerant Rhizopus microsporus DMKU 33 capable of producing l-lactic acid from liquefied cassava starch was isolated and characterized for its phylogenetic relationship and growth temperature and pH ranges. The concentrations of (NH4)2SO4, KH2PO4, MgSO4 and ZnSO4·7H2O in the fermentation medium was optimized for lactic acid production from liquefied cassava starch by Rhizopus microsporus...
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