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Direct effects of the enantiomers of the classical dopamine receptor ligand apomorphine on 5-HT 3 receptors were examined in N1E-115 neuroblastoma cells in whole-cell voltage clamp mode. R(-)-apomorphine (R(-)APO; 3-300 μM) evokes a small, transient inward ion current. At 30 μM, R(-)APO induces its maximum inward current, which is approximately 3% of the amplitude of the inward current induced...
In intact human T lymphocytes, voltage-gated K + [K(V)] channels and Ca 2+ -activated K + [K(Ca)] channels have been recorded using the patch clamp technique in the cell-attached configuration. The reversal potential of the voltage-gated current with high K + solution in the pipette gives a measure for the cell membrane potential (V M ). The open probability...
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