Search results for: Shiroh Onodera
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A critical step in producing the annual inactivated influenza vaccine is the development of high yield (hy) seed viruses by reassortment for improved growth in ovo. Although hy reassortants for type A influenza viruses have been developed for many years, hy B influenza reassortant virus development for vaccine production has proven difficult. In this study, we have developed fourteen hy influenza...
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The inner core of bacteriophage φ8 is capable of packaging and replicating the plus strands of the RNA genomic segments of the virus in vitro. The particles composed of proteins P1, P2, P4, and P7 can be assembled in cells of E. coli that carry plasmids with cDNA copies of genomic segment L. The gene arrangement on segment L was found to differ from that of other cystoviruses in that the gene for...
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Bacteriophage Φ8 has a genome of three dsRNA segments. It is able to acquire plasmid transcripts of cDNA copies of the genomic segments as replacements of its resident chromosomes. It is also able to effect recombination between the plasmid transcripts and the resident chromosomes. Depending upon the extent of sequence identity between the plasmid transcript and the resident chromosome, the recombination...
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The three dsRNA genomic segments of bacteriophage Φ13 were copied as cDNA and the nucleotide sequences were determined. The organization of the genome is similar to that of Φ6, and there is significant similarity in the amino acid sequences of the proteins of the polymerase complex and one of the membrane proteins, P6. There is little or no similarity in the nucleotide sequences. Several features...
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The three double-stranded RNA genomic segments of bacteriophage Φ8 were copied as cDNA, and their nucleotide sequences were determined. Although the organization of the genome is similar to that of Φ6, there is no similarity in either the nucleotide sequences or the amino acid sequences, with the exception of the motifs characteristic of viral RNA polymerases that are found in the presumptive polymerase...
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Bacteriophage φ6 has a genome of three segments of double-stranded RNA enclosed in a polyhedral procapsid. Plus strand transcripts of the segments are packaged in a serially dependent fashion in which S can package alone, M depends on S, and L depends on S and M. We have isolated a mutant form of the virus in the carrier state that has lost segment S. This finding presented an apparent anomaly with...
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Bacteriophage Φ6 has a genome of three segments of double-stranded RNA enclosed in a polyhedral procapsid. Packaging of individual segments is dependent upon unique packaging sequences near the 5' ends of the segments. We have prepared deletions in segments L and M that decrease their size by half. Phages with these deletions can be propagated on host strains carrying plasmids with complementing genes...
