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Biorepositories worldwide collect human serum samples and store them for future research. Currently, hundreds of biorepositories across the world store human serum samples in refrigerators, freezers, or liquid nitrogen without following any specific cryopreservation protocol. This method of storage is both expensive and potentially detrimental to the biospecimens. To decrease both cost of storage...
In an earlier paper [35], we examined the mutual interaction between the actin cytoskeleton and the cell membrane and explored the role this interaction plays during freeze/thaw. In this follow-up paper, we investigate the physical and chemical stresses induced by freeze/thaw and explore the different mechanisms of damage caused by these stresses. Our results showed that changes in cell volume during...
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