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BeWo choriocarcinoma cells were cultured onto solid microcarrier beads, packed into columns and superfused. Unidirectional influx of l -phenylalanine (l -phe) and l -leucine (l -leu) across the microvillous border of the cells was studied using a rapid paired-tracer dilution technique. Influx of l -phe andl -leu comprised both saturable and non-saturable components. K m values for l -phe and...
BeWo choriocarcinoma cells were cultured onto solid microcarrier beads, packed into syringe barrels and superfused. The unidirectional choline uptake across the microvillous membrane of the cells was measured by a rapid single-circulation paired-tracer dilution procedure using methyl[ 3 H]choline with d-[ 14 C]mannitol as the extracellular reference molecule. Choline influx was...
Image analysis of SDS-PAGE profiles of highly purified, paired maternal-facing (microvillous; MVM) and fetal-facing (basal; BM) plasmalemma membrane vesicles from six term human placentae showed that, while individual MVM or BM profiles were extremely reproducible, the two membrane populations were substantially different—although all of the seven major bands of molecular mass, 98.4, 79.4, 71.1, 45...
We here describe a novel procedure for culturing BeWo and JAr choriocarcinoma cells on solid microcarrier beads. The regime developed to stir the beads resulted, after about 10 days, in small aggregates of two to six beads covered with a layer of differentiated cells. Bead aggregates were packed into small columns and superfused, providing a dynamic in vitro system for studying the uptake mechanisms...