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Bull sperm plasma and outer acrosomal membranes were analyzed by SDS-PAGE. Analysis of the plasma membrane proteins revealed the presence of a 70 kDa band the prominence of which is enhanced after capacitation. This protein was found to bind to zona pellucida intact oocytes. PAGE analysis of outer acrosomal membrane proteins also reveals the presence of a 70 kDa band, but its prominence decreases...
Plasma and outer acrosomal membranes were extracted from bovine spermatozoa and used in an in vitro fusion assay. Fusion was revealed by monitoring the merging of lipids using the chlorophyll a-N,N'-dioctadecyloxacarbocyanine-p-toluene sulfonate (DCY) method [(1984) Biochim. Biophys. Acta 769. 531-542]. The requirement for capacitation, as well as the effects or pH, calcium and spermine, on membrane...
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