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Studies of the interactions between biological molecules are of highest importance in a vast number of areas of biomedicine and biotechnology. Today there are several different methods used for affinity studies such as surface film resonance, mass spectroscopy, ultracentrifugation, chromatography and electrophoresis and so on. Our approach utilizes GMR microfluidic biosensor, an emerging idea tool...
Giant magnetoresistance (GMR) is a quantum mechanical magnetoresistance effect observed in thin-film structures composed of alternating ferromagnetic and non-magnetic conductive layers. Today GMR has been used in various fields including detecting DNA hybridization and pathogenic microorganism[1], screening diagnostic biomarkers[2], detecting HBV genotypes[3], and so on. NT-proBNP is an established...
We report a novel microfluidic surface-enhanced Raman scattering (SERS) device, which is achieved by bonding a polydimethylsiloxane cap with a microchannel structure onto an SERS-active substrate composed of noble-metal covered silicon nanopillar forests. The silicon nanopillar forests are fabricated by using nanomaterial dots, which are introduced in oxygen-plasma bombardment of photoresist, as etching...
An integrated fluidic system has been fabricated, capable of separating a mixture of different bio-molecules into its components. It is composed of a filter and an actuator; the pressure generated by the actuator sustains the flow of the mixture through the filter. The actuator is made by stacking several layers of conductive polymer. Actuator strain in excess of 10% has been obtained, which corresponds...
To meet the requirements of infectious diseases identification, a sealed and fully enclosed cartridge with self-contained reagents was developed. The inlet and outlet ports of the cartridge are self-sealing. The waste produced during the diagnostic process was collected in a waste bag, which is enclosed in the cartridge. Two different types of waste bags were designed to reduce the overall thickness...
In this work, a novel device for batch separation of particles based on dielectrophoresis (DEP) is presented. The curved parts of serpentine micro-channel that causes performance deterioration in capillary electrophoresis (CE) due to the plug dispersion are effectively used in our design for dielectrophoretic separation. The top-and-bottom focusing electrodes are employed in the intermediate straight...
Current paper describes the fabrication and testing of a Lab-on-a-chip device using TMMF S2030 dry film resist for single cell analysis. The device typically consists of a bigger channel (20 mum deep), 3 smaller channels (2 mum deep) with embedded reservoir in between and a detector towards the downstream.Whole device has been fabricated on a standard glass wafer. Electrode configuration is the notable...
Current paper describes the testing, alignment and bonding of a PDMS-glass bonded microfluidic device used for single cell analysis. The channels were successfully tested using carboxylate modified polystyrene beads for any leakage. Polystyrene beads of 2 mum have been successfully trapped within the reservoir of the device by well known dielectrophoresis technique and the same technique will be used...
The fabrication of micro-channels in single-mode optical fibers is demonstrated using focused femtosecond laser processing and chemical etching. Straight line micro-channels are achieved based on a simple technique which overcomes limitations imposed by the fiber curved surface
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