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A simple HPLC method was developed to distinguish between 'poisonous' and 'non-poisonous' chemotypes of Ferula communis. The method was performed on a C 8 reverse phase analytical column using a binary eluent (aqueous TFA 0.01%-TFA 0.01% in acetonitrile) under gradient condition. The two chemotypes showed different fingerprints. The identification of five coumarins and eleven daucane derivatives...
Isocyclosporins are isomers of cyclosporins deriving from an N,O-acyl transfer occurring under acidic conditions. For the identification by 1 H NMR of isocyclosporins prepared in organic solvents the signal at 2.3 ppm assigned to three hydrogens of the NHCH 3 group is considered. Results reported here show that the 1 H NMR spectrum of isocyclosporin A prepared in aqueous acidic...
We have previously reported that treatment of CsA with aqueous HCl gives rise to the formation of a number of water-soluble compounds. Two of these were identified from their FAB-MS/MS spectra as open-chain nona- and decapeptides. We describe here the identification of two other main compounds deriving from the same treatment. Identification was rendered possible from the comparison of their FAB-MS/MS...
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