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Using the plastic-plate of rice seedling, we studied that phosphogypsum as acidifier in affected on the growth of rice seedling. It was shown that phosphogypsum could adjust soil pH value of into the pH range of rice seedling growing, which had increased effectively plant height, root length, dry weight, chlorophyll content and root activity of rice seedling. Phosphogypsum treatment mixed sulfuric...
Chlorpyrifos, a broad spectrum orgnaophosphorus insecticide, is extensively used in agricultural and household insect control, but the potential health effects associated human exposure to low levels is unclear, which has been subject of increasing concern in the last years. However, few studies about chlorpyrifos toxicity have been conducted in the model organism Caenorhabditis elegans, which can...
In order to fully explore and comprehensive utilization the resource of Dictyoploca japonica, the pupa protein was extracted form pupa of Dictyoploca japonica by sodium hydroxide in this study. The pH of the solubility of protein had been determined. The best condition of extracting pupa protein: the concentration of NaOH is 1.5%, with the temperature 70??, the time 3 h and the pupa powder should...
It's well known that irreversible cross-linked complexes between proteins, produced by formaldehyde interacting with free amino or amide groups to form Schiff s base, are formed in diabetes and aging patients. To identify such specific modified peptides as biomarkers for clinical diagnose of diabetes and AD, cross-linkage of formaldehyde with human serum albumin (HSA) was analyzed by LC/ESI/MS in...
Aligning protein sequences using a score matrix has became a routine but valuable method in modern biological research. However, alignment in "Twilight Zone" is still a challenging issue. New score matrices such as Position Specific Score Matrix and structural context-based matrix have been issued to deal with it, whereas generalized matrices are still useful in pairwise alignment and/or...
The neural stem cells were induced by low-level dopamine (DA). We allowed cells 72 h of differentiation before protein extraction. The global proteins of neural stem cells and dopaminergic neurons were extracted. In this study, the protein from cell lysat was rudiment separated by SDS-PAGE, and the gel strip was cut into several portions. The protein in each portion is digested by trypsin, and then...
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