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Conventional purification methods for hepatitis B core antigen (HBcAg) such as sucrose-gradient ultracentrifugation and size-exclusion chromatography are tedious and time-consuming. Therefore, a new approach using aqueous two-phase system (ATPS) was employed in this study to purify HBcAg from unclarified Escherichia coli feedstock. Phase inversion and separation are important factors in protein partitioning,...