Search results for: A.J. El Haj

Items from 1 to 20 out of 27 results

article

The influence of hydrostatic pressure on tissue engineered bone development

K.H.L. Neßler, J.R. Henstock, A.J. El Haj, S.L. Waters, more

Journal of Theoretical Biology > 2016 > 394 > C > 149-159

The hydrostatic pressure stimulation of an appropriately cell-seeded porous scaffold within a bioreactor is a promising method for engineering bone tissue external to the body. We propose a mathematical model, and employ a suite of candidate constitutive laws, to qualitatively describe the effect of applied hydrostatic pressure on the quantity of minerals deposited in such an experimental setup. By...

article

Evaluation of skeletal tissue repair, Part 2: Enhancement of skeletal tissue repair through dual-growth-factor-releasing hydrogels within an ex vivo chick femur defect model

E.L. Smith, J.M. Kanczler, D. Gothard, C.A. Roberts, more

Acta Biomaterialia > 2014 > 10 > 10 > 4197-4205

There is an unmet need for improved, effective tissue engineering strategies to replace or repair bone damaged through disease or injury. Recent research has focused on developing biomaterial scaffolds capable of spatially and temporally releasing combinations of bioactive growth factors, rather than individual molecules, to recapitulate repair pathways present in vivo. We have developed an ex vivo...

article

Evaluation of skeletal tissue repair, Part 1: Assessment of novel growth-factor-releasing hydrogels in an ex vivo chick femur defect model

E.L. Smith, J.M. Kanczler, D. Gothard, C.A. Roberts, more

Acta Biomaterialia > 2014 > 10 > 10 > 4186-4196

Current clinical treatments for skeletal conditions resulting in large-scale bone loss include autograft or allograft, both of which have limited effectiveness. In seeking to address bone regeneration, several tissue engineering strategies have come to the fore, including the development of growth factor releasing technologies and appropriate animal models to evaluate repair. Ex vivo models represent...

article

Cyclic hydrostatic pressure stimulates enhanced bone development in the foetal chick femur in vitro

J.R. Henstock, M. Rotherham, J.B. Rose, A.J. El Haj

Bone > 2013 > 53 > 2 > 468-477

Mechanical loading of bone and cartilage in vivo results in the generation of cyclic hydrostatic forces as bone compression is transduced to fluid pressure in the canalicular network and the joint synovium. It has therefore been suggested that hydrostatic pressure is an important stimulus by which osteochondral cells and their progenitors sense and respond to mechanical loading in vivo. In this study,...

article

Characterisation of myosin heavy chain gene variants in the fast and slow muscle fibres of gammarid amphipods

N.M. Whiteley, J.L. Magnay, S.J. McCleary, S. Khazraee Nia, more

Comparative Biochemistry and Physiology, Part A > 2010 > 157 > 2 > 116-122

Recent molecular work has revealed a large diversity of myosin heavy chain (MyHC) gene variants in the abdominal musculature of gammarid amphipods. An unusual truncated MyHC transcript from the loop 1 region (Variant A ₃ ) was consistently observed in multiple species and populations. The current study aimed to determine whether this MyHC variant is specific to a particular muscle fibre type,...

article

Hyperpolarization of Human Mesenchymal Stem Cells in Response to Magnetic Force

G.R. Kirkham, K.J. Elliot, A. Keramane, D.M. Salter, more

IEEE Transactions on NanoBioscience > 2010 > 9 > 1 > 71 - 74

Magnetic particle tagging techniques are currently being applied to tissue engineering applications such as controlled differentiation of mesenchymal stem cells (MSC). In order to define key mechanotransducers underpinning these applications, the electrophysiological responses of human MSCs (hMSC) have been investigated. Ferromagnetic microparticles were coated with L-arginyl-glycyl-L-aspartic acid...

article

Linking functional molecular variation with environmental gradients: Myosin gene diversity in a crustacean broadly distributed across variable thermal environments

J. Rock, J.L. Magnay, S. Beech, A.J. El Haj, more

Gene > 2009 > 437 > 1-2 > 60-70

To investigate the molecular basis of temperature adaptation in natural populations we used the candidate gene approach, targeting the myosin heavy chain (MyHC) gene. The functional effects of genetic variation in MyHC have been well characterised, and changes in the flexibility of the surface loops 1 and 2, caused by modulations in length, amino acid composition and charge can play an important role...

article

CORRELATING CELL MORPHOLOGY AND OSTEOID MINERALIZATION RELATIVE TO STRAIN PROFILE FOR BONE TISSUE ENGINEERING APPLICATIONS

M.A. Wood, Y. Yang, E. Baas, D.O. Meredith, more

Journal of Biomechanics > 2008 > 41 > Supplement 1 > S160

article

Development of Superparamagnetic Iron Oxide Nanoparticles (SPIONS) for Translation to Clinical Applications

Meng Meng Lin, Do Kyung Kim, A.J. El Haj, J. Dobson

IEEE Transactions on NanoBioscience > 2008 > 7 > 4 > 298 - 305

Superparamagnetic iron oxide nanoparticles (SPIONs) have attract a great deal of interest in biomedical research and clinical applications over the past decades. Taking advantage the fact that SPIONs only exhibit magnetic properties in the presence of an applied magnetic field, they have been used in both in vitro magnetic separation and in vivo applications such as hyperthermia (HT), magnetic drug...

article

Characterisation of scaffold architecture and tendons using optical coherence tomography and polarisation-sensitive optical coherence tomography

Y. Yang, P.O. Bagnaninchi, B. Hu, K. Hampson, more

IET Optoelectronics > 2008 > 2 > 5 > 188 - 194

Scaffolds play an important role in the generation of functional tissues using tissue-engineering techniques. To generate highly organised tissue, scaffolds must have specific internal and external architectures. Here, optical coherence tomography (OCT) is exploited to characterise the architectures of various scaffolds, in particular scaffolds which have been fabricated to support the formation of...

article

Characterizing the efficacy of calcium channel agonist-release strategies for bone tissue engineering applications

M.A. Wood, S. Hughes, Y. Yang, A.J. El Haj

Journal of Controlled Release > 2006 > 112 > 1 > 96-102

We have previously reported on the use of Bay K8644-release strategies in combination with perfusion–compression bioreactor systems for up regulating bone formation in three-dimensional PLLA scaffolds. Here we report on the analysis of Bay activity following its release from our PLLA scaffolds over the culture period imposed in our tissue engineering protocol using UV spectroscopy in combination with...

article

In vitro bone regeneration and micro-mechanical strain in mechanoactive scaffolds

E. Baas, J.H. Kuiper, Y. Yang, M.A. Wood, more

Journal of Biomechanics > 2006 > 39 > Supplement 1 > S219

article

Development of optical imaging for on-line monitoring in tissue engineering

A.J. El Haj, R. Wang, P. Baganinchi, N. Mafulli, more

Journal of Biomechanics > 2006 > 39 > Supplement 1 > S447

article

In vivo tracking of stem cells using magnetic tagging in a nude mouse model

S. Wolbank, H. Sura, J. Dobson, M. van Griensven, more

Journal of Biomechanics > 2006 > 39 > Supplement 1 > S447

article

FT-IR microspectroscopy as a tool to assess lung cancer cells response to chemotherapy

J. Sulé-Suso, D. Skingsley, G.D. Sockalingum, A. Kohler, more

Vibrational Spectroscopy > 2005 > 38 > 1-2 > 179-184

Lung cancer is a disease associated with a poor prognosis. One of the reasons for this is the lack of effective treatment. Chemotherapy, together with surgery and radiotherapy, is one of the main treatments for this disease. However, it is not possible at present to determine which tumours would be more sensitive to a given drug or combination of drugs. In this study, FT-IR microspectroscopy was used...

article

Temperature-dependent developmental variation in lobster muscle myosin heavy chain isoforms

J.L. Magnay, J.M. Holmes, D.M. Neil, A.J. El Haj

Gene > 2003 > 316 > Complete > 119-126

The temperature- and developmental-regulation of myosin heavy chain (MyHC) expression and primary sequence was investigated in the abdominal musculature of developing Homarus gammarus larvae acclimated to 10, 14 and 19+/-1 ^o C. MyHC loop 1 (ATP binding) and loop 2 (actin binding) regions were sequenced and compared. The deduced amino acid sequence of MyHC loop 1 showed a development-related...

article

Comparison of the variable loop regions of myosin heavy chain genes from Antarctic and temperate isopods

J.M. Holmes, N.M. Whiteley, J.L. Magnay, A.J. El Haj

Comparative Biochemistry and Physiology, Part B > 2002 > 131 > 3 > 349-359

The evolutionary adaptations of functional genes to life at low temperatures are not well characterised in marine and fresh water invertebrates. Temperature has been shown to affect the functional characteristics of fish muscles, with changes in the velocity of shortening and ATPase activity being associated with myosin heavy chain (MyHC) isoform composition and the structure of the surface loop regions...

article

Effects of temperature on specific dynamic action and protein synthesis rates in the Baltic isopod crustacean, Saduria entomon

R.F. Robertson, A.J. El-Haj, A. Clarke, E.W. Taylor

Journal of Experimental Marine Biology and Ecology > 2001 > 262 > 1 > 113-129

The mean rate of oxygen consumption in fasted Saduria entomon increased between 4 ^o C and 13 ^o C with a Q ₁ ₀ of 2.33. After feeding, rates of oxygen uptake increased significantly by two- to threefold at both temperatures. However, the magnitude of the 'specific dynamic action of food' (SDA) response varied with temperature. At 4 ^o C, the magnitude was 45.5+/-4...

article

Nicotinic regulation of c-fos and osteopontin expression in human-derived osteoblast-like cells and human trabecular bone organ culture

L.M. Walker, M.R. Preston, J.L. Magnay, P.B.M. Thomas, more

Bone > 2001 > 28 > 6 > 603-608

Long-term in vivo studies have highlighted smoking as a risk factor in postmenopausal osteoporosis, bone fracture incidence, and increased nonunion rates. In contrast, there are few data postulating the effects of smoking at the cellular level in human skeletal tissue. In this study, we present novel evidence demonstrating that the nicotinic receptor α4 subunit is present in human primary bone cells...

article

Protein synthesis and specific dynamic action in crustaceans: effects of temperature

N.M. Whiteley, R.F. Robertson, J. Meagor, A.J. El Haj, more

Comparative Biochemistry and Physiology, Part A > 2001 > 128 > 3 > 593-604

Temperature influences the specific dynamic action (SDA), or rise in oxygen uptake rate after feeding, in eurythermal and stenothermal crustaceans by changing the timing and the magnitude of the response. Intra-specific studies on the eurythermal crab, Carcinus maenas, show that a reduction in acclimation temperature is associated with a decrease in SDA magnitude, resulting from an increase in SDA...

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Search results for: A.J. El Haj

The influence of hydrostatic pressure on tissue engineered bone development

Evaluation of skeletal tissue repair, Part 2: Enhancement of skeletal tissue repair through dual-growth-factor-releasing hydrogels within an ex vivo chick femur defect model

Evaluation of skeletal tissue repair, Part 1: Assessment of novel growth-factor-releasing hydrogels in an ex vivo chick femur defect model

Cyclic hydrostatic pressure stimulates enhanced bone development in the foetal chick femur in vitro

Characterisation of myosin heavy chain gene variants in the fast and slow muscle fibres of gammarid amphipods

Hyperpolarization of Human Mesenchymal Stem Cells in Response to Magnetic Force

Linking functional molecular variation with environmental gradients: Myosin gene diversity in a crustacean broadly distributed across variable thermal environments

CORRELATING CELL MORPHOLOGY AND OSTEOID MINERALIZATION RELATIVE TO STRAIN PROFILE FOR BONE TISSUE ENGINEERING APPLICATIONS

Development of Superparamagnetic Iron Oxide Nanoparticles (SPIONS) for Translation to Clinical Applications

Characterisation of scaffold architecture and tendons using optical coherence tomography and polarisation-sensitive optical coherence tomography

Characterizing the efficacy of calcium channel agonist-release strategies for bone tissue engineering applications

In vitro bone regeneration and micro-mechanical strain in mechanoactive scaffolds

Development of optical imaging for on-line monitoring in tissue engineering

In vivo tracking of stem cells using magnetic tagging in a nude mouse model

FT-IR microspectroscopy as a tool to assess lung cancer cells response to chemotherapy

Temperature-dependent developmental variation in lobster muscle myosin heavy chain isoforms

Comparison of the variable loop regions of myosin heavy chain genes from Antarctic and temperate isopods

Effects of temperature on specific dynamic action and protein synthesis rates in the Baltic isopod crustacean, Saduria entomon

Nicotinic regulation of c-fos and osteopontin expression in human-derived osteoblast-like cells and human trabecular bone organ culture

Protein synthesis and specific dynamic action in crustaceans: effects of temperature

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