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Introduction: Although the multilineage potential of human processed lipoaspirate has previously been demonstrated, few studies exist utilizing mouse adipose-derived mesenchymal cells (ADMSCs) in vitro or in vivo. Here, we develop a method for osteogenic differentiation of ADMSCs in vitro and utilize them to repair calvarial defects in vivo.Methods: ADMSCs were harvested from 3 week old female FVB...
Introduction: Data indicates that TGF-betas may play an important role in craniosynostosis. In a murine organ culture model, we previously demonstrated that blocking TGF-beta signaling leads to pathologic suture patency. In this study, we investigated both the osteogenic properties of TGF-beta1 and determined whether adenoviral overexpression of TGF-beta1 in a normally patent sagittal (SAG) suture...
Introduction: Similarities between skeletal formation and repair have led to the theory that adult fracture repair may parallel development. We hypothesize that since the mandible is derived from cranial neural crest (CNC) and forms via intramembranous ossification, non-stabilized mandibular fractures will heal in a similar fashion.Methods: Non-stabilized osteotomies were created in the right hemi-mandible...
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