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We have used an interspecific backcross to map the chromosomal locations of the mouse homologs of the genes encoding myr 3 (unconventional myosin from rat), myr 4, and myr 5, members of the myosin superfamily of motor proteins. The mouse loci have been namedMyo1e, Myo1c,andMyo9b;they map to chromosomes 9 (MGD position 41), 11 (MGD position 46), and 8 (MGD position 31.5), respectively.
We have isolated two cDNAs that encode putative myosin I heavy chains by polymerase chain reaction amplification of brain cDNA with degenerate oligodeoxynucleotide primers representing myosin I-speeific conserved amino acid sequences. We report the complete deduced amino acid sequence of one of these cDNAs. The sequence is most similar to those of the avian and bovine brush border myosin Is, with...
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