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Muscle glycogen exists as acid-insoluble (AIG) and acid-soluble (ASG) forms, with AIG levels reported in most recent studies in humans to be the most responsive to exercise and refeeding. Because the muscle samples in these studies were not homogenized to extract glycogen, such homogenization-free protocols might have resulted in a suboptimal yield of ASG. Our goal, therefore, was to determine whether...
Most recent studies on the physiology of proglycogen and macroglycogen in skeletal muscles have adopted a homogenization-free acid extraction protocol to separate these 2 pools of glycogen. The purposes of this study were to determine (a) whether this protocol is suitable; (b) if the acid-insoluble glycogen fraction corresponds to proglycogen; and (c) if this fraction accounts for most of the changes...