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The third reading frame of the envelope gene from HIV-1 codes for a protein homologous to the human selenoprotein glutathione peroxidase (GPX). Cells stably or transiently transfected with a HIV-1 GPX construct are protected against the loss of the mitochondrial transmembrane potential and subsequent cell death induced by exogenous reactive oxygen species (ROS) as well as mitochondrion-generated ROS...
Glucocorticoids play an important role in the negative selection of thymocytes. We examined the action of dexamethasone during B cell development and activation by monitoring proliferation, viability, cell cycle, expression of surface IgM (sIgM) and alkaline phosphatase (ALP), used as a marker of B lymphocyte activation.Cell viability was assessed by Trypan blue exclusion and, ALP activity was determined...
The aim of this study was to investigate requirements for de novo protein synthesis in apoptotic process, which is a controversial topic. We analysed the effects of cycloheximide (CHX), an inhibitor of macromolecular synthesis, on B lymphocytes apoptosis.B cells were purified from murine spleen cells. Cell viability was determined by morphological examination after FDA and BET staining. Apoptosis...
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