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Dictyostelium discoideum is of considerable interest as an expression system for the production of proteins of high value. The cultivation of this social amoeba is not as easy as that of other common microbial expression systems. Wildtype strains grow on bacteria. Mutant strains growing on axenic media reach cell densities of 1-2x10 7 ml -1 when cultivated in commonly used complex...
An improved fully synthetic medium was presented recently [Process Biochem. (in press)] based on the synthetic medium formulated by Franke and Kessin in 1977 [Proc. Natl. Acad. Sci. USA 74 (1977) 2157]. This novel medium was called SIH instead of the classical FM medium. The improved medium leads to even higher cell densities, a more balanced uptake of amino acids and a better utilisation of glucose...
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