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This paper describes the development and validation of an isothermal GC-FID method for the assay of tributyl phosphate in a phospholipid emulsion. The emulsion is used as a topical ointment to deliver Triton X-100, a spermicide. The tributyl phosphate is added to the emulsion as a plasticizer or softening agent. The chromatographic conditions of the method employ a J&W DB-Wax capillary column...