Search results for: Salvador Peirú

Items from 1 to 15 out of 15 results

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Industrial uses of phospholipases: current state and future applications

Sebastián Cerminati, Luciana Paoletti, Andrés Aguirre, Salvador Peirú, more

Applied Microbiology and Biotechnology > 2019 > 103 > 6 > 2571-2582

Phospholipids play a central role in all living organisms. Phospholipases, the enzymes aimed at modifying phospholipids, are consequently widespread in nature and play diverse roles, from lipid metabolism and cellular signaling in eukaryotes to virulence and nutrient acquisition in microbes. Phospholipases catalyze the hydrolysis of one or more ester or phosphodiester bonds of glycerophospholipids...

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The βγ-crystallin domain of Lysinibacillus sphaericus phosphatidylinositol phospholipase C plays a central role in protein stability

Sebastián Cerminati, Luciana Paoletti, Salvador Peirú, Hugo G. Menzella, more

Applied Microbiology and Biotechnology > 2018 > 102 > 16 > 6997-7005

βγ-crystallin has emerged as a superfamily of structurally homologous proteins with representatives across all domains of life. A major portion of this superfamily is constituted by microbial members. This superfamily has also been recognized as a novel group of Ca²⁺-binding proteins with a large diversity and variable properties in Ca²⁺ binding and stability. We have recently described a new phosphatidylinositol...

article

The production, properties, and applications of thermostable steryl glucosidases

Andres Aguirre, Florencia Eberhardt, Guillermo Hails, Sebastian Cerminati, more

World Journal of Microbiology and Biotechnology > 2018 > 34 > 3 > 1-9

Extremophilic microorganisms are a rich source of enzymes, the enzymes which can serve as industrial catalysts that can withstand harsh processing conditions. An example is thermostable β-glucosidases that are addressing a challenging problem in the biodiesel industry: removing steryl glucosides (SGs) from biodiesel. Steryl glucosidases (SGases) must be tolerant to heat and solvents in order to function...

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Pilot-scale process development for low-cost production of a thermostable biodiesel refining enzyme in Escherichia coli

Florencia Eberhardt, Andres Aguirre, Luciana Paoletti, Guillermo Hails, more

Bioprocess and Biosystems Engineering > 2018 > 41 > 4 > 555-564

Biodiesels produced from vegetable oils have a major quality problem due to the presence of steryl glucosides (SGs), which form precipitates that clog filters and cause engine failures. Recently, we described an enzymatic process for removing SGs from biodiesel. However, industrial adoption of this technology was hindered by the cost of the steryl glucosidase (SGase) enzyme used. Here we report the...

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B. cereus phospholipase C engineering for efficient degumming of vegetable oil

Claudia Elena, Sebastian Cerminati, Pablo Ravasi, Rodolfo Rasia, more

Process Biochemistry > 2017 > 54 > C > 67-72

Enzymatic phospholipid removal (degumming) is a fast-growing and environmentally friendly process for vegetable oil refining. Type C phospholipases (PLC) are the preferred enzymes since they provide an extra yield in the oil recovery.Bacillus cereus PLC can hydrolyze phosphatidylcholine (PC) but has a limited efficiency at removing phosphatidylethanolamine (PE), which together represent ∼70% of the...

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Development of a highly efficient oil degumming process using a novel phosphatidylinositol-specific phospholipase C enzyme

Sebastián Cerminati, Florencia Eberhardt, Claudia E. Elena, Salvador Peirú, more

Applied Microbiology and Biotechnology > 2017 > 101 > 11 > 4471-4479

Enzymatic degumming using phospholipase C (PLC) enzymes may be used in environmentally friendly processes with improved oil recovery yields. In this work, phosphatidylinositol-specific phospholipase C (PIPLC) candidates obtained from an in silico analysis were evaluated for oil degumming. A PIPLC from Lysinibacillus sphaericus was shown to efficiently remove phosphatidylinositol from crude oil, and...

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Pichia pastoris engineering for the production of a modified phospholipase C

Claudia Elena, Pablo Ravasi, Sebastián Cerminati, Salvador Peiru, more

Process Biochemistry > 2016 > 51 > 12 > 1935-1944

Crude vegetable oils are refined to remove impurities that adversely impact in their stability, color and flavor. In recent years, enzymatic degumming methods using phospholipase C (PLC) enzymes provide an environmentally friendly process for phospholipids removal with improved oil recovery yields.In this study, Pichia pastoris was used as the expression system for the production of PLC-Y, a modified...

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Strain engineering and process optimization for enhancing the production of a thermostable steryl glucosidase in Escherichia coli

Florencia Eberhardt, Andres Aguirre, Hugo G. Menzella, Salvador Peiru

Journal of Industrial Microbiology & Biotechnology > 2017 > 44 > 1 > 141-147

Biodiesels produced from transesterification of vegetable oils have a major problem in quality due to the presence of precipitates, which are mostly composed of steryl glucosides (SGs). We have recently described an enzymatic method for the efficient removal of SGs from biodiesel, based on the activity of a thermostable β-glycosidase from Thermococcus litoralis. In the present work, we describe the...

article

High-level production of Bacillus cereus phospholipase C in Corynebacterium glutamicum

Pablo Ravasi, Mauricio Braia, Florencia Eberhardt, Claudia Elena, more

Journal of Biotechnology > 2015 > 216 > Complete > 142-148

Enzymatic oil degumming (removal of phospholipids) using phospholipase C (PLC) is a well-established and environmentally friendly process for vegetable oil refining. In this work, we report the production of recombinant Bacillus cereus PLC in Corynebacterium glutamicum ATCC 13869 in a high cell density fermentation process and its performance in soybean oil degumming. A final concentration of 5.5g/L...

article

A Fluorometric Enzymatic Assay for Quantification of Steryl Glucosides in Biodiesel

Andrés Aguirre, Matías Cabruja, Rodolfo Cabrera, Florencia Eberhardt, more

Journal of the American Oil Chemists' Society > 2015 > 92 > 1 > 47-53

Steryl glucosides (SG) are common contaminants in biodiesel that form precipitates, which form and cause problems due to fouling during transport and storage. Therefore, their quantification is necessary to assess the quality of this fuel. The methods currently available for SG analysis require expensive instrumentation, need a previous concentration step by solid-phase extraction (SPE) or are of...

article

An industrial scale process for the enzymatic removal of steryl glucosides from biodiesel

Salvador Peiru, Andres Aguirre, Florencia Eberhardt, Mauricio Braia, more

Biotechnology for Biofuels > 2015 > 8 > 1 > 1-12

Background Biodiesels produced from transesterification of vegetable oils have a major quality problem due to the presence of precipitates, which need to be removed to avoid clogging of filters and engine failures. These precipitates have been reported to be mostly composed of steryl glucosides (SGs), but so far industrial cost-effective methods to remove these compounds are not available. Here we...

article

Enzymatic hydrolysis of steryl glucosides, major contaminants of vegetable oil-derived biodiesel

Andres Aguirre, Salvador Peiru, Florencia Eberhardt, Leandro Vetcher, more

Applied Microbiology and Biotechnology > 2014 > 98 > 9 > 4033-4040

Biodiesels are mostly produced from lipid transesterification of vegetable oils, including those from soybean, jatropha, palm, rapeseed, sunflower, and others. Unfortunately, transesterification of oil produces various unwanted side products, including steryl glucosides (SG), which precipitate and need to be removed to avoid clogging of filters and engine failures. So far, efficient and cost-effective...

article

Recombinant approaches to large polyketide molecules as potential drugs

Salvador Peirú, Hugo C. Gramajo, Hugo G. Menzella

Drug Discovery Today: Technologies > 2010 > 7 > 2 > e105-e113

Combinatorial biosynthesis of polyketide synthases through genetic engineering should provide complex compounds as drug development candidates. Different strategies are currently being evaluated to create libraries of new compounds. The number of different compounds in such libraries and the quantities that can be made using combinatorial strategies will determine the impact of this technology on...

article

Characterization of the Heterodimeric MegBIIa:MegBIIb Aldo-Keto Reductase Involved in the Biosynthesis of l -Mycarose from Micromonospora megalomicea ^†

Salvador Peirú, Eduardo Rodríguez, Chau Q. Tran, John R. Carney, more

Biochemistry > 2007 > 46 > 27 > 8100 - 8109

article

Characterization of an oxaloacetate decarboxylase that belongs to the malic enzyme family

Pablo D. Sender, Mauricio G. Martı́n, Salvador Peirú, Christian Magni

FEBS Letters > 2004 > 570 > 1-3 > 217-222

The citM gene from Lactococcus lactis CRL264 was demonstrated to encode for an oxaloacetate decarboxylase. The enzyme exhibits high levels of similarity to malic enzymes (MEs) from other organisms. CitM was expressed in Escherichia coli, purified and its oxaloacetate decarboxylase activity was demonstrated by biochemical and genetic studies. The highest oxaloacetate decarboxylation activity was found...

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