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On Biorex 70 ion exchanger at neutral pH the histones H3 and H4 are usually eluted by 4Mguanidinium chloride (gdm Cl). In order to protect cysteines and methionines from oxidation we systematically added 2-mercaptoethanol to the elution buffer. This resulted in the two histones being unexpectedly eluted together at around 1Mgdm Cl. The use of a shallower gradient resulted in a division in the peak...