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This study was performed to elucidate the potential of human platelet lysate (huPL) as an alternative to xeno-free media for culturing mesenchymal stem cells (MSCs). In this work, Wharton's jelly-derived MSCs (WJ-MSCs; n = 5) were isolated, characterized, and cultured in huPL derived from type O platelets reconstituted with type AB platelet-poor plasma (huPL–ABO). WJ-MSCs from five donors were cultured...