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Seven new sites leading to increased β-lactam susceptibility were identified and mapped in Staphylococcus aureus by Tn551 or Tn917 mediated insertional inactivation. Their effect was more pronounced in methicillin resistant than in susceptible strains. Except for inserts each in SmaI-K and SmaI-B, all were located on SmaI-A, which covers 25% of the staphylococcal chromosome. The physical position...