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The gene pcpA that encodes a novel pneumococcal choline-binding protein has been cloned and characterized. Northern blot analysis revealed that pcpA is expressed during the exponential phase of growth of pneumococci as a monocistronic transcript of about 2.3 kb. The transcription start site has been located 132 bp upstream of the start codon and the proposed -35 and -10 boxes that are highly similar...
Three genes homologous to cspA, which encodes the major secretable protein of Clostridium beijerinckii NCIB 8052 have been cloned and sequenced. The Csp proteins showed the typical modular structure of cell-wall associated proteins and, that found in the choline-binding proteins of Streptococcus pneumoniae. The variable number of repeats that constitute the C-terminal choline-binding domain suggests...
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