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Complementary DNAs (cDNAs) encoding the human metabotropic glutamate receptor 4 (mGluR4) and human metabotropic glutamate receptor 7 (mGluR7) were isolated from a human cerebellum or fetal brain cDNA library by hybridization with probes that were generated by PCR and degenerate primers derived from rat mGluR sequences. The human mGluR4 and mGluR7 receptors are proteins of 912 and 915 amino acid residues...
The present invention provides a method for increasing expression of low molecular weight polypeptides which are produced by recombinant DNA transformation of prokaryotic host cells. This invention allows the direct expression of these polypeptides without fusion to an otherwise expressible protein and requires only the addition of a single amino acid to the sequence of the polypeptide product of...
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