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Literary data on the dissociation constants of lipid ionizable groups were revised in the light of our recent finding that the standard approach used in the determination of pK values from experimental titration curves needs corrections for cation adsorption. Such corrections were made for both the apparent and the intrinsic pK of two anionic lipids, phosphatidylserine and phosphatidylglycerol. ...
A well-known approach used in the determination of the apparent pK (pK a ) value of ionizable groups is re-examined for the case of highly charged lipid membranes in monovalent electrolytes with specifically adsorbing cations. It is shown that cation adsorption leads to a significant decrease in the maximal charge density measured at neutral pH using a charge density (σ) vs. pH curve. As...
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