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Escherichia coli changes its metabolism in response to environmental circumstances, and metabolic adaptations are evident in hungry bacteria growing slowly in glucose-limited chemostats. The role of isocitrate lyase (AceA) was examined in E. coli growing under glucose limitation. AceA activity was elevated in a strain-dependent manner in the commonly used E. coli K-12 laboratory strains MG1655 and...
The ptsG gene encodes the high-affinity glucose receptor component of the PEP:glucose phosphotransferase system. PtsG is the major glucose transporter in Escherichia coli under glucose-excess conditions but its regulation under glucose limitation or anaerobiosis is poorly defined. Using a ptsG-lacZ transcriptional fusion, ptsG expression was found to peak with low (micromolar) external glucose levels...
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