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Plant regeneration via somatic embryogenesis and organogenesis was achieved in leaf base and ovary culture of the Croatian endemic Iris adriatica Trinajstić ex Mitić. Callus induction from leaf base explants occurred in the dark on three media with MS mineral solution containing 4.52 μM dichlorophenoxyacetic acid (2,4-D), 4.83 μM naphthaleneacetic acid (NAA), 0.46 μM kinetin (Kin), 5% sucrose and...
The aim of the study was to determine the onset, duration and termination of the ragweed pollen season; intradiurnal, daily and monthly pollen variation, and possible differences in the occurrence and concentration of ragweed pollen according to sampling sites between 2002 and 2003. The study was conducted at three sampling sites in central Croatia over two pollen seasons (2002 and 2003) using the...
Although intraspecies researches within the black pine (Pinus nigra Arnold) have a long tradition, the intraspecies taxonomy, classification and chorology are still unclear. Among the numerous reasons that have caused this situation the most important are: the absence of a study that would completely cover the whole range of this species, the impossibility of connection of results of the existing...
Employing the volumetric method by use of a Hirst sampler, a total of 71,286 pollen grains, as many as 94.20% of them allergenic, were recorded in the air samples from the city of Zagreb during the 2002 pollen season. Among identified pollen of 35 plant species/genera/families, 23 were allergenic: Taxus/Juniperus, Alnus sp., Fraxinus sp., Betula sp., Corylus sp., Poaceae, Urticaceae, Artemisia sp...
There are many unsolved taxonomic problems at the intraspecific level in the genus Thalictrum (Ranunculaceae), which may be clarified using molecular systematics methods. No molecular systematics methods have been applied yet to the genus Thalictrum; this study analyzes different leaf tissue preservation and DNA isolation techniques, and the applicability of RAPD. A modified DNA isolation procedure...
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