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Histochemistry (percentage number of three type fibres and their cross-sectional area) and changes in hardness, rheological properties (elastic and viscous moduli), and structural elements (mean fibre cross-sectional area and thickness of endomysium) of four wild boar muscles of different ages: biceps femoris (BF), semimembranosus (SM), quadriceps femoris (QF), and longissimus (L) subjected to effective...
A multiplex PCR assay designed by Bansal [1996] was applied in a routine screening test of a meat-processing environment aimed at detection of Listeria monocytogenes and species of the genus Listeria. Listeria spp. strains yielded a single 938-bp product indicating presence of 16S rRNA conservative sequence typical of the genus, whereas L. monocytogenes strains yielded not only the 938-bp product...
The efficacy of confirmatory tests recommended by a PrPN EN ISO 11290-1:1999 standard for identification of Listeria spp. and L. monocytogenes strains in food products was examined in our studies. Confirmatory assays consisted of catalase and motility (a characteristic 'umbrella- -shape' outgrowth) tests for Listeria spp. as well as hemolytic activity and sugar fermentation (D-xylose and L-rhamnose)...
Two advanced methods for identification of Listeria monocytogenes strains: a commercial miniaturised biochemical test - API®LISTERIA (bioMérieux) and polymerase chain reaction (PCR) adapted to the in vitro amplification of the iap gene fragment (453 bp), were used in comparative studies. A total of 63 strains suspected, based on morphological criteria, to belong to L. monocytogenes species were subjected...
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