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Celem pracy było stworzenie dla celów dydaktycznych trójwymiarowego modelu oraz animacji przedstawiającej zasadę działania mikroskopu konfokalnego. Dokonano tego na przykładzie mikroskopu Olympus LEXT OLS4000 przy pomocy programu Cinema 4D, Adobe After Effects oraz Adobe Premiere Pro. Przedstawiono całą koncepcję tworzenia animacji. Następnie opisano proces tworzenia elementów mikroskopu wraz z ich...
The dependence of actin-activated ATPase activity and myosin filament structure on Ca 2+ -concentration in the range between pCa 7.5 and pCa 4.6 have been studied. Rabbit skeletal muscle myosin with dephosphorylated regulatory light chains column-purified with DEAE-Sephadex A-50 for the removal of minor proteins and actin without regulatory proteins have been used. A considerable increase...
Dephosphorylated and phosphorylated heavy meromyosin, fluorescently labeled with 1,5-IAEDANS attached at the SH 1 group, was introduced into myosin-free ghost fibres and the polarized fluorescence of the bound label was measured. The results depended on whether the divalent cation binding sites on heavy meromyosin were saturated with Mg 2+ or Ca 2+ . The calculated...
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