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BLV is an agent of enzootic bovine leukaemia (EBL), an infectious disease affecting cattle worldwide. BLV infection has been associated with immune system disorders and discrepancies in the cytokine network. The significance of dendritic cells in the pathogenesis of BLV infection is largely unknown, but considering their fundamental role in immune response it may be crucial. Dcs precursors were isolated...
The aim of the study was to develop an in situ PCR (IS-PCR) method for detection of bovine leukemia virus (BLV) in cell cultures. Samples from five BLV positive and five BLV negative cows were collected and dendritic cells (DCs) from blood, bone marrow, spleen, and lymph node were cultured. Cultures prepared from healthy animals were infected with BLV. After two weeks, the cells were tested by nested...
Determination of telomerase activity was performed with the use of telomerase PCR ELISA method in blood lymphocytes, dendritic cells, and cells isolated from lymphatic organs. Telomerase activity was detected in almost all investigated samples, but relative telomerase activity was on different levels. The highest activity of telomerase was determined in human tumour cell lines and FLK-BLV cells, but...
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