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Work reported here shows the outcome of improvements made to a published PCR-RFLP approach for fish species identification. The objective of the improved method was to replace the gel-electrophoretic steps for fragment separation, detection and analysis, by employing a chip-based capillary electrophoresis (CE) system. Fragment resolution on the system was sensitive, with detection of small fragments...
Species-specific real-time PCR (TaqMan) assays were developed for detection of beef, pork, lamb, chicken and turkey. Assays were developed around small (amplicons <150 base pairs) regions of the mitochondrial cytochrome b (cytb) gene. Speciation was achieved using species-specific primers. For detection purposes, two TaqMan probes were developed; the first was specific to the mammalian species...
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