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Ci-MRF is the sole myogenic regulatory factor (MRF) of the ascidian Ciona intestinalis, an invertebrate chordate. In order to investigate its properties we developed a simple in vivo assay based on misexpressing Ci-MRF in the notochord of Ciona embryos. We used this assay to examine the roles of three structural motifs that are conserved among MRFs: an alanine–threonine (Ala–Thr) dipeptide of the...
J. Neurochem. (2010) 115, 984–993.
AbstractVesicular acetylcholine transporter (VAChT; TC 2.A.1.2.13) mediates storage of acetylcholine (ACh) by synaptic vesicles. A three‐dimensional homology model of VAChT is available, but the binding sites for ACh and the allosteric inhibitor (−)‐trans‐2‐(4‐phenylpiperidino)cyclohexanol (vesamicol) are unknown. In previous work, mutations of invariant W331 in...
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