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Reaction dynamics of a chloride ion pump protein, halorhodopsin (HR), from Natronomonas pharaonis (N. pharaonis) (NpHR) was studied by the pulsed-laser-induced transient grating (TG) method. A detailed investigation of the TG signal revealed that there is a spectrally silent diffusion process besides the absorption-observable reaction dynamics. We interpreted these dynamics in terms of release, diffusion,...
Natronomonas pharaonis halorhodopsin (pHR) is an archaeal rhodopsin functioning as an inward-directed, light-driven Cl− pump. To characterize the electrophysiological features of the Cl− pump activity of pHR, we expressed pHR in Xenopus laevis oocytes and analyzed its photoinduced Cl− pump activity using the two-electrode voltage-clamp technique. Photoinduced outward currents were observed only in...
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