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The level of intracellular diadenosine 5′, 5′′′-P1,P4-tetraphosphate (Ap4A) increases several fold in mammalian cells treated with non-cytotoxic doses of interstrand DNA-crosslinking agents such as mitomycin C. It is also increased in cells lacking DNA repair proteins including XRCC1, PARP1, APTX and FANCG, while >50-fold increases (up to around 25μM) are achieved in repair mutants exposed to mitomycin...
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