Search results for: Marilena Manea

Items from 1 to 20 out of 26 results

article

Molecular characterization of the β‐amyloid(4‐10) epitope of plaque specific Aβ antibodies by affinity‐mass spectrometry using alanine site mutation

Raluca Ștefănescu, Loredana Lupu, Marilena Manea, Roxana E. Iacob, more

Journal of Peptide Science > 24 > 1 > n/a - n/a

Alzheimer disease is a neurodegenerative disease affecting an increasing number of patients worldwide. Current therapeutic strategies are directed to molecules capable to block the aggregation of the β‐amyloid(1‐42) (Aβ) peptide and its shorter naturally occurring peptide fragments into toxic oligomers and amyloid fibrils. Aβ‐specific antibodies have been recently developed as powerful antiaggregation...

article

Improved in vivo antitumor effect of a daunorubicin - GnRH-III bioconjugate modified by apoptosis inducing agent butyric acid on colorectal carcinoma bearing mice

Bence Kapuvári, Rózsa Hegedüs, Ákos Schulcz, Marilena Manea, more

Investigational New Drugs > 2016 > 34 > 4 > 416-423

Summary Compared to classical chemotherapy, peptide-based drug targeting is a promising therapeutic approach for cancer, which can provide increased selectivity and decreased side effects to anticancer drugs. Among various homing devices, gonadotropin-releasing hormone-III (GnRH-III) peptide represents a suitable targeting moiety, in particular in the treatment of hormone independent tumors that highly...

article

Time- and pH-Dependent Copper Binding to Aβ(1–16) Peptide: An Electrospray Ionization-Mass Spectrometric Approach

Marilena Manea, Gitta Schlosser, Manuela Murariu

International Journal of Peptide Research and Therapeutics > 2015 > 21 > 1 > 125-131

An elevated concentration of copper ions in the brain of Alzheimer’s disease patients has been reported in many studies and might be associated with an increased aggregation of β-amyloid (Aβ) peptides. In the present work, the interaction with copper ions of a model β-amyloid peptide, Aβ(1–16), was investigated by electrospray ionization-mass spectrometry (ESI–MS) at two pH values, 7.4 and 6.6, as...

article

Gonadotropin-releasing hormone receptors as molecular therapeutic targets in prostate cancer: Current options and emerging strategies

Patrizia Limonta, Marilena Manea

Cancer Treatment Reviews > 2013 > 39 > 6 > 647-663

Prostate cancer is androgen-dependent in its early stages and androgen deprivation therapy represents the most effective first-line therapeutic approach. However, after an initial remission, prostate cancer progresses towards the castration resistant prostate cancer (CRPC) stage, with increased malignancy and resistance to conventional chemotherapy.Pituitary gonadotropin-releasing hormone receptors...

article

Synthesis, enzymatic stability and in vitro cytostatic effect of Daunorubicin-GnRH-III derivative dimers

Verena Natalie Schreier, Gábor Mező, Erika Orbán, Claudia Dürr, more

Bioorganic & Medicinal Chemistry Letters > 2013 > 23 > 7 > 2145-2150

Bioconjugates containing chemotherapeutic agents attached to peptide hormones, such as gonadotropin-releasing hormone (GnRH), are developed as drug delivery systems for targeted cancer chemotherapy. We report here the synthesis and biochemical characterization of disulfide bond-linked dimeric bioconjugates in which daunorubicin was coupled via an oxime linkage to aminooxyacetylated GnRH-III ([Glp-His-Trp-Ser-His-Asp-Trp-Lys(DauAoa-Cys)-Pro-Gly-NH...

article

Fully automated chip-based nanoelectrospray combined with electron transfer dissociation for high throughput top-down proteomics

Corina Flangea, Catalin Schiopu, Florina Capitan, Cristina Mosoarca, more

Open Chemistry > 2013 > 11 > 1 > 25-34

The conventional protocol for protein identification by electrospray ionization mass spectrometry (MS) is based on enzymatic digestion which renders peptides to be analyzed by liquid chromatography-MS and collision-induced dissociation (CID) multistage MS, in the so-called bottom-up approach. Though this method has brought a significant progress to the field, many limitations, among which, the low...

article

Fully automated chip-based nanoelectrospray combined with electron transfer dissociation for high throughput top-down proteomics

Corina Flangea, Catalin Schiopu, Florina Capitan, Cristina Mosoarca, more

Open Chemistry > 2013 > 11 > 1 > 25-34

article

Fully automated chip-based nanoelectrospray combined with electron transfer dissociation for high throughput top-down proteomics

Corina Flangea, Catalin Schiopu, Florina Capitan, Cristina Mosoarca, more

Central European Journal of Chemistry > 2013 > 11 > 1 > 25-34

article

Epitope structure and binding affinity of single chain llama anti‐β‐amyloid antibodies revealed by proteolytic excision affinity‐mass spectrometry

Gabriela Paraschiv, Cécile Vincke, Paulina Czaplewska, Marilena Manea, more

Journal of Molecular Recognition > 26 > 1 > 1 - 9

ß‐Amyloid (Aß) immunotherapy has become a promising strategy for reducing the level of Aß in brain. New immunological approaches have been recently proposed for rapid, early diagnosis, and molecular treatment of neurodegenerative diseases related to Alzheimer's Disease (AD). The combination of proteolytic epitope excision and extraction and mass spectrometry using digestion with various proteases...

article

Enhanced cellular uptake and in vitro antitumor activity of short-chain fatty acid acylated daunorubicin–GnRH-III bioconjugates

Rózsa Hegedüs, Marilena Manea, Erika Orbán, Ildikó Szabó, more

European Journal of Medicinal Chemistry > 2012 > 56 > Complete > 155-165

Here we report on the synthesis and biochemical characterization (enzymatic stability, cellular uptake, in vitro antitumor activity, membrane interaction and GnRH-receptor binding affinity) of novel short-chain fatty acid (SCFA) acylated daunorubicin–GnRH-III bioconjugates, which may serve as drug delivery systems for targeted cancer chemotherapy. Ser in position 4 of GnRH-III was replaced by Lys,...

article

GnRH-III based multifunctional drug delivery systems containing daunorubicin and methotrexate

Ulrike Leurs, Eszter Lajkó, Gábor Mező, Erika Orbán, more

European Journal of Medicinal Chemistry > 2012 > 52 > Complete > 173-183

Here we report on the design, synthesis and biochemical characterization of multifunctional bioconjugates containing two chemotherapeutic agents, daunorubicin and methotrexate, coupled to the GnRH-III decapeptide, which served as a targeting moiety. This represents a possible approach to increase the receptor mediated tumor targeting and consequently the cytostatic effect of anticancer drug-peptide...

article

Interaction structure of the complex between neuroprotective factor humanin and Alzheimer's β‐amyloid peptide revealed by affinity mass spectrometry and molecular modeling

Madalina Maftei, Xiaodan Tian, Marilena Manea, Thomas E. Exner, more

Journal of Peptide Science > 18 > 6 > 373 - 382

Humanin (HN) is a linear 24‐aa peptide recently detected in human Alzheimer's disease (AD) brain. HN specifically inhibits neuronal cell death in vitro induced by ß‐amyloid (Aß) peptides and by amyloid precursor protein and its gene mutations in familial AD, thereby representing a potential therapeutic lead structure for AD; however, its molecular mechanism of action is not well understood. We report...

article

Design, synthesis, in vitro stability and cytostatic effect of multifunctional anticancer drug‐bioconjugates containing GnRH‐III as a targeting moiety

Ulrike Leurs, Gábor Mező, Erika Orbán, Peter Öhlschläger, more

Biopolymers > 98 > 1 > 1 - 10

Bioconjugates containing the GnRH‐III hormone decapeptide as a targeting moiety are able to deliver chemotherapeutic agents specifically to cancer cells expressing GnRH receptors, thereby increasing their local efficacy while limiting the peripheral toxicity. However, the number of GnRH receptors on cancer cells is limited and they desensitize under continuous hormone treatment. A possible approach...

article

Anthracycline-GnRH derivative bioconjugates with different linkages: Synthesis, in vitro drug release and cytostatic effect

Pascal Schlage, Gábor Mező, Erika Orbán, Szilvia Bősze, more

Journal of Controlled Release > 2011 > 156 > 2 > 170-178

To increase the selectivity and consequently to minimize the side effects of chemotherapeutic agents, receptor mediated tumor targeting approaches have been developed. In the present work, various anthracycline-GnRH derivative bioconjugates were synthesized with the aim of investigating the influence of (i) different anthracycline anticancer drugs, (ii) different linkages between the targeting moiety...

article

A New Daunomycin–Peptide Conjugate: Synthesis, Characterization and the Effect on the Protein Expression Profile of HL-60 Cells in Vitro

Erika Orbán, Marilena Manea, Andreas Marquadt, Zoltán Bánóczi, more

Bioconjugate Chemistry > 2011 > 22 > 10 > 2154 - 2165

article

Enhanced Enzymatic Stability and Antitumor Activity of Daunorubicin-GnRH-III Bioconjugates Modified in Position 4

Marilena Manea, Ulrike Leurs, Erika Orbán, Zsuzsa Baranyai, more

Bioconjugate Chemistry > 2011 > 22 > 7 > 1320 - 1329

article

In vitro degradation and antitumor activity of oxime bond-linked daunorubicin–GnRH-III bioconjugates and DNA-binding properties of daunorubicin–amino acid metabolites

Erika Orbán, Gábor Mező, Pascal Schlage, Gabriella Csík, more

Amino Acids > 2011 > 41 > 2 > 469-483

Bioconjugates with receptor-mediated tumor-targeting functions and carrying cytotoxic agents should enable the specific delivery of chemotherapeutics to malignant tissues, thus increasing their local efficacy while limiting the peripheral toxicity. In the present study, gonadotropin-releasing hormone III (GnRH-III; Glp-His-Trp-Ser-His-Asp-Trp-Lys-Pro-Gly-NH₂) was employed as a targeting moiety to...

article

Efficient synthesis of an (aminooxy) acetylated‐somatostatin derivative using (aminooxy)acetic acid as a ‘carbonyl capture’ reagent

Gábor Mezö, Ildikó Szabó, István Kertész, Rózsa Hegedüs, more

Journal of Peptide Science > 17 > 1 > 39 - 46

Owing to the high chemoselectivity between an aminooxy function and a carbonyl group, oxime ligation is one of the most preferred procedures for the preparation of peptide conjugates. However, the sensitivity of (aminooxy)acetylated peptides to ketones and aldehydes makes their synthesis and storage difficult. In our study, we established the efficient synthesis of an (aminooxy)acetylated‐somatostatin...

article

Interaction of β-amyloid(1-40) peptide with pairs of metal ions: An electrospray ion trap mass spectrometric model study

Gabi Drochioiu, Marilena Manea, Mihaela Dragusanu, Manuela Murariu, more

Biophysical Chemistry > 2009 > 144 > 1-2 > 9-20

The stoichiometries and the affinity toward simple and paired metal ions of synthetic amyloid-β(1-40) peptide (Aβ1-40) were investigated by electrospray ion trap mass spectrometry (ESI-MS), circular dichroism (CD), and atomic force microscopy (AFM). The results lead to the working hypothesis that pH-dependent metal binding to Aβ1-40 may induce conformational changes, which affect the affinity toward...

article

Functional Ubiquitin Conjugates with Lysine-ε-Amino-Specific Linkage by Thioether Ligation of Cysteinyl-Ubiquitin Peptide Building Blocks

Ji Eun Jung, Hans-Peter Wollscheid, Andreas Marquardt, Marilena Manea, more

Bioconjugate Chemistry > 2009 > 20 > 6 > 1152 - 1162

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Search results for: Marilena Manea

Molecular characterization of the β‐amyloid(4‐10) epitope of plaque specific Aβ antibodies by affinity‐mass spectrometry using alanine site mutation

Improved in vivo antitumor effect of a daunorubicin - GnRH-III bioconjugate modified by apoptosis inducing agent butyric acid on colorectal carcinoma bearing mice

Time- and pH-Dependent Copper Binding to Aβ(1–16) Peptide: An Electrospray Ionization-Mass Spectrometric Approach

Gonadotropin-releasing hormone receptors as molecular therapeutic targets in prostate cancer: Current options and emerging strategies

Synthesis, enzymatic stability and in vitro cytostatic effect of Daunorubicin-GnRH-III derivative dimers

Fully automated chip-based nanoelectrospray combined with electron transfer dissociation for high throughput top-down proteomics

Fully automated chip-based nanoelectrospray combined with electron transfer dissociation for high throughput top-down proteomics

Fully automated chip-based nanoelectrospray combined with electron transfer dissociation for high throughput top-down proteomics

Epitope structure and binding affinity of single chain llama anti‐β‐amyloid antibodies revealed by proteolytic excision affinity‐mass spectrometry

Enhanced cellular uptake and in vitro antitumor activity of short-chain fatty acid acylated daunorubicin–GnRH-III bioconjugates

GnRH-III based multifunctional drug delivery systems containing daunorubicin and methotrexate

Interaction structure of the complex between neuroprotective factor humanin and Alzheimer's β‐amyloid peptide revealed by affinity mass spectrometry and molecular modeling

Design, synthesis, in vitro stability and cytostatic effect of multifunctional anticancer drug‐bioconjugates containing GnRH‐III as a targeting moiety

Anthracycline-GnRH derivative bioconjugates with different linkages: Synthesis, in vitro drug release and cytostatic effect

A New Daunomycin–Peptide Conjugate: Synthesis, Characterization and the Effect on the Protein Expression Profile of HL-60 Cells in Vitro

Enhanced Enzymatic Stability and Antitumor Activity of Daunorubicin-GnRH-III Bioconjugates Modified in Position 4

In vitro degradation and antitumor activity of oxime bond-linked daunorubicin–GnRH-III bioconjugates and DNA-binding properties of daunorubicin–amino acid metabolites

Efficient synthesis of an (aminooxy) acetylated‐somatostatin derivative using (aminooxy)acetic acid as a ‘carbonyl capture’ reagent

Interaction of β-amyloid(1-40) peptide with pairs of metal ions: An electrospray ion trap mass spectrometric model study

Functional Ubiquitin Conjugates with Lysine-ε-Amino-Specific Linkage by Thioether Ligation of Cysteinyl-Ubiquitin Peptide Building Blocks

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