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Cytochrome P450 4F3 (CYP4F3) catalyzes the inactivation of leukotriene B4 by ω-oxidation in human neutrophils. Here, we examined the mRNA and protein expression of the CYP4F3 in HL-60 cells, an acute promyelocytic leukemia cell line, in the presence of hydroquinone(HQ). We found hydroquinone led to a decrease in cell proliferation and a increase in cell apoptosis in a dose-dependent and time-dependent...
MicroRNAs (miRNAs) are small noncoding RNAs which regulate gene expression. In this research, we used the Multiz alignments data of 44 Vertebrates to retrieve syntenic homolog sequences. And we identified 37 members of miR-1302 family among 71 potential homolog sequences. Then we analyzed the 37 homologs of hsa-mir-1302s by RepeatMasker and found all of these sequences are derived from MER53 elements...
The partial sequence of the psbA gene from Cladosiphon okamuranus was amplified by PCR and their sequences were analysed. Neighbor-joining method was used to build phylogenetic trees according to the psbA sequence among 11 species, C. okamuranus included, that are affiliated to the algal order Ectocarpales. The results showed that the nucleotide sequence is 737 bp and the sequence shares high homology...
The entomopathogenic fungus Beauveria bassiana, an important biological control agent of lepidopteron, is also the key pathogen to cause epizootic among the silkworm, Bombyx mori, a kind of insect very economically important as producer of silk in China. At present, very little is known about the isolates that are associated with the endemic or epizootic phases of this disease agent. Random amplified...
In the ABC model, MADs-box proteins play an important role in floral development. As one of the MADs-box proteins, PI behaves as B function organ-identity genes acting in the second and third whorls of the flower to control petal and stamen identity. To find genes involved in the floral transition of pineapple guava, we cloned the full length cDNA encoding a MADs-box protein in Coolidge pineapple...
Single-cell gel electrophoresis (SCGE) is a useful technical tool to detect DNA damages. In this work, tobacco plants (Nicotiana tabacum var. xanthi) were cultivated in different concentrations of nitrobenzene, and the DNA damages were analyzed by using SCGE. The results showed that the DNA damages increased with increasing nitrobenzene concentrations. Results of the present study suggest that nitrobenzene...
In this study, random amplified polymorphic DNA (RAPD) analysis was used to estimate the genetic diversity among six Oratosquilla oratoria populations: Dalian (DL), Wafangdian (WF), Zhuanghe (ZH), Donggang (DG), Suizhong (SZ) in Liaoning province and Qingdao (QD) in Shandong province. 18 random primers for RAPD analyzing generated 256 bands, out of which 209 were polymorphic loci. The number of bands...
Study on microbial community structure and diversity in freshwater is important for exploiting the relationship of microbial community and its habitats, predicting and monitoring the ecological changing of contaminated water habitat. The retrieval of 16SrDNA genes directly from the environment has made it possible to estimate the phylogenetic diversity of a natural bacterial community without preceding...
We determined the nucleotide sequence of a portion of the duck enteritis virus (DEV) strain CHv which was suspected to be the UL10 gene(GenBank accession no EU195100.), encoding glycoprotein M(gM). The UL10 gene was cloned and sequenced, which was composed of 1230 nucleotides encoding 409 amino acids, with a predicted molecular mass of 45.752 kDa. We calculated the codon usage bias in the newly identified...
The interaction between nanomaterials and genomes is getting more and more attention. Such studies are getting involved into several fields: environment safety, food safety, nanobiotechnology and drug development. Direct interaction between nanoparticles and DNA in vitro has been reported in several studies, but data for in vivo binding between genomic DNA and nanoparticles are rare. In this study,...
Endoplasmic reticulum stress(ERS)is a kind of sub-cellular pathological process which result from calcium disorderliness and protein improper folding. And endoplasmic reticulum stress induced apoptosis is a novel pathway which different from the mitochondria-initiated intrinsic pathway and the death receptor-triggered extrinsic pathway. Trichosanthin (TCS), a type I ribosome-inactivating protein,...
A new severe disease with the record of the cucumber bacterial white spot was observed on cucumber seedlings in a greenhouse in Liaoning province of China since 2007. The spot of the disease is round or subround, membranous in the middle and halos encompass. Bacterial ooze is not found around the disease position. Relative studies are taken on Biolog tests and 16S rDNA gene sequence for CU-PV 08....
An algicidal bacterium was isolated from Lake Taihu during the bloom of Microcystis in 2009. It was Gram-negative, oxidase-negative, and produced red-colored colonies on agar media. The strain was tentatively identified as Serratia marcescens by means of physiological, biochemical reactions and homology analysis based on 16S rDNA. The bacteria could degrade Microcystis aeruginosa effectively. When...
Eighty eight accessions including eighty six of Solanum melongena and two wild related species (Solanum integrifolium and Solanum torvum) were used to analyze the genetic variation with 23 SSR primers. And 20 primers were successfully amplified, of which 19 showed polymorphism with a total of 68 detected alleles. The number of alleles per primer ranged from 2 to 6, with a mean of 3.6. Among 19 polymorphism...
The glycoprotein I (gI) gene homologue of duck plague virus (DPV) was cloned by degenerate polymerase chain reaction (PCR) and sequenced. DPV gI gene open reading frame (ORF) was 1116 bp in length and its primary translation product was a polypeptide of 371 amino acids long. Comparison with other herpesvirus revealed higher similarity, had a close evolution relationship with members of the genus Mardivirus...
To detect and identify of Enterohemorrhagic Escherichia coli O157:H7, the gene rfbE of O157:H7 was detected as the aim gene and the Agilent 2100 bioanalyzer was used as an analysis tool, which was compared with the conventional agarose gel electrophoresis. Finally, it was known that the rfbE gene was successful in detection of O157:H7. And the bioanalyzer was as 10 times sensitive as the agrouse gel...
Based on a newly proposed 3-D graphical representation of DNA sequences, we perform sequences analysis on essential genes among different species. The results show that this new model can help to exhibit the intrinsic features of DNA sequence. From this representation, 24 variables are exploited as quantitative descriptors of DNA sequences, their applications in essential genes as well as their complementary...
β-ketoacyl-ACP synthetase II (KAS II) which catalyzes the elongation of 16:0-ACP to 18:0-ACP, determines the ratio of palmitic acid (16:0) to stearic acid (18:0). Using Rapid Amplification of cDNA Ends (RACE) and a peanut cDNA library we had previously constructed, we isolated a 1867bp cDNA of the KAS II gene containing a 1212bp complete open reading fragment (ORF). Sequence analysis revealed that...
This study compared the whole composition of microbial community in continuous-flow (MBR) and batch-fed (discontinuous) (MSBR) aerobic membrane bioreactors using high-density universal 16S rRNA Microarray, which includes 506,944 probes targeted to 8935 clusters in 16S rRNA gene sequences. The Microarray results shown both MBR and MSBR had high microbial diversity. 1126 and 1002 bacterial subfamilies...
15 bacterial strains with a capacity of poly-phosphate were isolated from enrichment culture of the soil sample collected from the phosphate fertilizer factory of Honghu City in Hubei province, of which one strain named as HS-P7 has the strongest effects of poly-phosphate. Ammonium molybdate spectrophotometric method was used to measure its ability of poly-phosphate. The results showed that phosphorus...
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