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Single-stranded DNA (ssDNA) oligonucleotide in solution, or that is immobilized onto a surface to create a biosensor, can be used as a selective probe to bind to a complementary single-stranded sequence. Fluorescence enhancement of thiazole orange (TO) occurs when the dye intercalates into double-stranded DNA (dsDNA). TO dye has been covalently attached to probe oligonucleotides (homopolymer and mixed...
We have developed a novel generation of optical bioaffinity sensors for ultra trace analysis. These sensors are based on luminescence generation in the evanescent field of high-refractive-index single-mode planar waveguides. With the waveguiding layers and the grating parameters chosen, very sharp discrimination of bulk against surface-confined excitation, in combination with high excitation intensities...
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