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In our previous studies, we have developed a glucose indictor used for continuous glucose concentration. We used this indicator to construct a glucose sensor that can be used for minimum-invasively monitoring blood glucose concentration for diabetics. It has been understood that this indicator is pH sensitive, making it less reliable in sensing the intracellular glucose concentration using fluorescence...
We present our findings on using coherent anti-Stokes Raman scattering (CARS) microscopy to image brain tissue slices. Compared with other modalities such as confocal and two-photon laser scanning microscopy, CARS microscopy offers chemical selectivity with high sensitivity without the need for any labeling agents. CARS microscopy uses two laser frequencies, whose energy difference is tuned to target...
A novel method was developed for the determination of DNA. The method was based on the mechanism of interaction between functionalized nanoparticle ZnS and deoxyribonucleic acid (DNA). Fluorescence intensity of ZnS is quenched by DNA at medium pH. The optimal condition was chosen for DNA and fluorescence spectrometry was used. Linear relationship between the quenched fluorescence intensity and the...
We present the development and performance characteristics of a free-space fluorescence tomography system. The imaging system can capture complete angle projections of photons propagating through tissue in transillumination using a CCD camera. Experimental data on imaging lung cancer are presented. Overall, this imaging approach can offer unprecedented imaging performance in fluorescence molecular...
We present the development and performance characteristics of a free-space fluorescence tomography system. The imaging system can capture complete angle projections of photons propagating through tissue in transillumination using a CCD camera. Experimental data on imaging lung cancer are presented. Overall, this imaging approach can offer unprecedented imaging performance in fluorescence molecular...
The association of CNVs with disease is of interest in medical genetics. This paper makes use single nucleotide polymorphism (SNP) to infer copy number variation (CNV). A microrray technology known as SNP-CGH (SNP-comparative genomic hybridization) is used to detect DNA CNV. Current SNP-CGH arrays contain > 100, 000 probes. The Illumina BeadChip has been designed for whole genome SNP genotyping...
Frequency domain fluorescence technique forms a strong platform for many sensitive biomedical imaging applications. In this paper, detection of human genomic DNA sample (in capillary tube) fluorescence was carried out with frequency-domain (FD) imaging technique. This sensitive imaging technique may provide a cost effective DNA fluorescence detection by minimizing the sample volume. Also, initial...
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