The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
We determined the nucleotide sequence of a portion of the duck enteritis virus (DEV) strain CHv which was suspected to be the UL10 gene(GenBank accession no EU195100.), encoding glycoprotein M(gM). The UL10 gene was cloned and sequenced, which was composed of 1230 nucleotides encoding 409 amino acids, with a predicted molecular mass of 45.752 kDa. We calculated the codon usage bias in the newly identified...
This work investigated the potential use of benzoic acid (BA)-based immunoadsorbent, a hydrophobic adsorbent, for autoantibodies selective adsorption therapy. The adsorbent was assessed through chromatographer and batch system assays in terms of adsorption capacity, selectivity for both polycloneantibodies in normal human plasma and pathogenic antibodies in patients' sera. For human immunoglobulin...
Glycoprotein K encoded by the DEV-UL53 gene is one of the DEV envelope glycoproteins. Like most members of the alphaherpesvirinae, the genes in the UL region of its genome are well conserved, which means that the major DEV envelope glycoprotein K possessing similar functions of envelope glycoprotein K of alphaherpesvirinae plays an essential role in viral replication and cell fusion. Glycoprotein...
This project focuses on the improvement of NDV production technology and innovation of the NDV vaccine preparation process with cell culture technology. Methods: To use primary chick embryo cell culture system in preparation of NDV anti-tumor vaccine. Results:In the new vaccine development process, the focused on maintaining and improving NDV titer while reducing the residual bovine serum. So that...
Endoplasmic reticulum stress(ERS)is a kind of sub-cellular pathological process which result from calcium disorderliness and protein improper folding. And endoplasmic reticulum stress induced apoptosis is a novel pathway which different from the mitochondria-initiated intrinsic pathway and the death receptor-triggered extrinsic pathway. Trichosanthin (TCS), a type I ribosome-inactivating protein,...
Non-mitogenic form of recombinant Human acidic fibroblast growth factor (nrhaFGF) retained the cardio and neuro-protective characters of the wild-type aFGF while decreased the potential side effects like mitogenesis activity. In this paper, nrhaFGF was successfully expressed in Escherichia coli and yielded about 5% of the total soluble protein in the 10L fermentation tank. the succeedent chromatography,...
A variety of xenogeneic and allogeneic extracellular matrix (ECM) scaffolds have been promising in various tissue engineering applications, such as the porcine small intestinal submucosa. Yet no particular scaffolds have been optimal for vocal fold regeneration. The decellularized human umbilical vein (HUV) is a novel allogeneic scaffold that has shown some promise for cardiovascular tissue engineering...
Objective: To construct the recombinant eukaryotic expression vector pcDNA3.1-CEMP1 containing the Human Cementum Protein 1 (hrCEMP1) by PCR and T/A cloning and establish NIH3T3 fibroblast cellline that stably expressing it. Methods: A pair of primers specific for amplifying the DNA fragment encoding hrCEMP1 were designed and synthesized. hrCEMP1 was inserted to the proper sites of vector pcDNA3.1...
Background: CD44v6 is a splice variant of CD44 (CD44v) could probably promote cancer cells to adhere to vascular endothelium and base membranes and enhancing the invasion and metastasis of colonic carcinomas. Heat shock protein 72 (HSP72) as a molecular chaperone has been confirmed to overexpress in epithelial carcinoma cells. There may be a possible correlation between the expression of HSP72 and...
Cystathionine β-synthase deficiency is an inborn error of metabolism that is biochemically characterized by severe hyperhomocysteinemia and homocystinuria. Numerous cystathionine β-synthase mutants have so far been found in patients with cystathionine β-synthase deficiency. In this study, we use the cross-impact analysis to model the cystathionine βsynthase mutants with their clinical endpoints, and...
Lipoprotein lipase (LPL) of Tianfu meat goose was cloned and sequenced using a RT-PCR approach. The nucleotide sequence covered 468 bp with an open reading frame of 155 amino acids. Alignment analysis indicated that the nucleotide sequences are highly conserved to other species studied including chicken, domestic guinea pig, house mouse, Norway rat, cattle, cow and goat, and the homologies are 92...
The analysis on codon usage bias of Us4 gene of Duck Plague Virus (DPV) may provide a basis for understanding the evolution and pathogenesis of DPV and for selecting appropriate host expression systems to improve the expression of target genes in vivo and in vitro. In this study, the synonymous codon usage in the Us4 gene of DPV and 17 reference herpesviruses have been investigated . The results reveals...
Mitochondria are a vital organelle of eukaryotic cells. Many diseases are caused by the mitochondrial gene mutation , therefore, there is an urgent requirement to explore the proteins of mitochondrion. In this paper we have created a list of 1086 human mitochondrial genes by combining 13 datasets from publications or genomic experiments. Then we created a functional linkage network among mitochondrial...
We explore whether antisense oligodeoxy-nucleotides (ASODN) targeting survivin gene can reverse apoptotic resistance induced by cisplatin in human lung adenocarcinoma cells. A549 / CDDP cell lines were cultured routinely with RPMI-1640 medium. Survivin ASODN mediated by cytofectin was transfected into the A549 / CDDP cells. The influence of ASODN transfection on apoptosis was determined by Diphenylamine...
scFv (single-chain Fv fragment) is the gene engineered antibody employed widely in at present. Recently there have been some progress on colorectal cancer diagnosis and treatment-oriented with scFv as carrier, but it is differential on affinity and anti-tumor activity of scFv with that of derived factor. Proper linker could make scFv have more suitable biological activity on clinical application....
Nitroreductase gene NOR1 is possibly involved in the chemical carcinogenesis of hepatic cancer and nasopharyngeal carcinoma. We have demonstrted that NOR1 overexpression could convert monofunction alalkylating agent, CB1954 ([5-aziridin-1-yl]-2,4-dinitrobenzamide) into a toxic form by reduction of the 4-nitro group of CB1954 and enhancing cell killing in nasopharyngeal carcinoma(NPC) cell line CNE...
Chiral pesticides are used widely in the world, and at present, older racemic products are being replaced by enantiopure products. Pesticides as xenobiotic released into environment impose a great stress on nontarget organisms. Traditionally it is a necessary procedure for pesticides to have a registration based on toxicological data from nontarget organism, but until now ecological risk assessment...
This study aimed to study the immune activity of glutamate dehydrogenase (GDH) of streptococcus suis serotype 2 and detect its activity. METHODS: The GDH gene was cloned and sequenced. Recombinant GDH protein was expressed in a prokaryotic expression system and purified by affinity chromatography. Immunocompetenc of the resulting protein was analyzed by western blotting with anti-GDH monoclonal antibody...
Low-power laser irradiation (LPLI), a non-damage physical therapy, which has been used clinically for decades of years, was shown to promote cell proliferation and prevent apoptosis. Apoptosis is a contributing pathophysiological mechanism of Alzheimer's disease (AD). However, whether LPLI could prevent apoptosis in AD model is not clear. In this study, we demonstrated for the first time that LPLI...
Comparison of a newly found enzyme with its structural homologues results in the prediction of probable regulatory binding sites. Since serine racemases are found to be promising targets for the treatment of disorders related to NMDAR dysfunction, the identification of the structural similarities between serine racemases and their structural homologues might provide structural insights for rational...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.