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Background: Although clinical significance of serum p53 Abs (s-p53 Abs) in cancer patients has been studied during the past few years, how to detect it effectively is a crucial issue. Methods: wild-type p53 protein (wt p53) and phage-displayed peptide were prepared. Three types of enzymelinked immunosorbent assays (ELISA) methods were constructed and a hybrid antigen-ELISA approach was attempted....
Atherosclerosis is a multifactorial disease involving a lot of genes and proteins recruited throughout its manifestation. The present study represents an integrative effort, coupling the results of a bioinformatic analysis based on microarray data of atherosclerotic aortic lesions of apoE knockout mice, a model widely used in atherosclerosis research, together with gene expression measurements of...
Ginger (Zingiber officinale) is used as a cooking spice in the diet in many parts of the world. Recent research has found ginger to have various pharmacological properties due to a variety of active constituents, including shogaols and gingerols. The aim of the present study was to assess the effect of alcohol extract of Zingiber officinale on the fractions of serum protein in mice. For this purpose,...
Protein-Protein Interactions (PPIs) play a very important role in many cellular processes and a variety of experimental approaches have been developed for their identification. These approaches however suffer from high error rates. Recently, computational methods have been employed to assist for the prediction. A common problem with the applied computational methods is that they either result in low...
In this study, we present experimental, modeling and simulation results for affinity binding between human immunoglobulin G (IgG) and protein A using an AT-cut quartz crystal microbalance (QCM) biosensor. A multilayer configuration transmission line model was built to predict the IgG-Protein A binding affinity on the QCM surface. The influence of antibody-antigen bonding effect on resonance frequency...
This paper proposes a novel method for analyzing similarities of protein sequences based on features generated from the hydropathy properties of amino acid sequences. At first using the hydropathy characteristics, the protein sequences are converted into fixed-dimensional feature vectors. Based on these feature vectors, we analyze nine ND5 proteins and construct a phylogenic tree as an application...
Lots of studies have focused on the structure and function of high-quality human metabolic networks. Recently, hub-based centrality is used to analysis of human metabolic network core. However, it is clear that this specific centrality measure is not sufficient alone. In the present paper, by comparing 10 different centrality measures with their application to human metabolic network core, be similar...
Eukaryotic genomes are packaged into chromatin by histone proteins whose chemical modification can profoundly influence gene expression. The histone modifications often act in combinations, which exert different effects on gene expression. Although a number of experimental techniques and data analysis methods have been developed to study histone modifications, it is still very difficult to identify...
Synchrotron radiation is used in ATR-Far-IR investigations of pig ear skin. Characteristic protein and water bands are assigned. The results are compared to low-wavenumber Raman spectra. IR-imaging is performed to show the distribution of water, proteins and lipids for pig ear tissue slices.
The causation of cancer often involves the joint deregulation of multiple biological processes. Thus, it is interesting to extract multi-function features of cancer genes and study their functional coordination involved in tumorigenesis. Here, based on Gene Ontology, we proposed a heuristics strategy to extract multi-function features which are significantly overrepresented with cancer genes. We showed...
Purpose To investigate the expression of small G protein R-Ras in breast cancer and to explore the significance of R-Ras, expression in the carcinogensis and development of breast carcinoma, and we also analysed the correlation of R-Ras expressions with ER, PR. Methods Real time quantitative reverse transcription polymerase chain reaction (RQ-RT-PCR) technique was applied for detecting R-Ras expression...
The identification of disease microRNAs is vital for understanding the pathogenesis of diseases at the molecular level, and is critical for designing specific molecular tools for diagnosis, treatment and prevention. However, one major issue in microRNA studies is the lack of bioinformatics methods to accurately predict microRNA-disease associations. Herein, we proposed an approach to infer microRNA-disease...
The sensitive detection of molecular biomarkers in clinical samples is crucially important in disease diagnosis. In this study, we propose and develop aptamer microarray platform combined with novel sol-gel technology to identify low abundance targets from complex solution, such as serum. Due to nanoporous structure of sol-gel, it entraps a large number of active aptamers and allows aptamers to bind...
Prion protein (PrP) is probably the only known infectious protein that possesses chameleon-like features, as reflected not only in its conformation but also in its function. Cellular PrP (PrPC), rich in α-helical structures, is believed to be functionally involved in neurotransmitter metabolism, immune cell activation, cell adhesion, signal transduction, copper metabolism, antioxidant activity, and...
Summary form only given. Deoxyribonucleic acid (DNA) has become one of the most examined molecules on the planet. Scientist around the world have been trying to unravel its secrets for many purposes. For example, genetic information is currently used to raise better plants and animals, create enhanced pharmaceuticals for humans, and for gene therapy in medicine. Science as a whole has benefited from...
Currently, the size of biological databases has increased significantly with the growing number of users and the rate of queries where some databases are of terabyte size. Hence, there is an increasing need to access databases at the fastest possible rate. Where biologists are concerned, the need is more of a means to fast, scalable and accuracy searching in biological databases. This may seem to...
Cytochrome P450 4F3 (CYP4F3) catalyzes the inactivation of leukotriene B4 by ω-oxidation in human neutrophils. Here, we examined the mRNA and protein expression of the CYP4F3 in HL-60 cells, an acute promyelocytic leukemia cell line, in the presence of hydroquinone(HQ). We found hydroquinone led to a decrease in cell proliferation and a increase in cell apoptosis in a dose-dependent and time-dependent...
We tested the hypothesis that shedding of membrane FasL is a mechanism for downregulating FasL/Fas signaling and both membrane and soluble FasL are involved in cardiomyocyte hypoxia/ reoxygenation (H/R) injury. We examined the relative importance of mitochondrial damage and direct cleavage of the executioner caspases by activated initiator caspase-8 in the propagation of FasL/Fas signaling activated...
Curcumin, the most active constituent of turmeric, is currently one of the major anticance new drugs and has been reported to enhance Heme Oxygenase-1(HO-1) in human brain when oxidative stress occur. Keeping the balance of HO-1 and HO-2 plays an important role in the brain. However, few researches study the effects of curcumin on the two isoenzymes of HO. Here we investigated the effects of curcumin...
Since diseases might be related with each other, systematically assessing their relationships could provide us novel insight into their mechanisms. One of the most important methods to study diseases' relationships is to calculate their phenotype similarity scores based on the text and clinical synopsis parts of their records in the OMIM database. However, as demonstrated in this paper, the similarity...
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