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A variety of xenogeneic and allogeneic extracellular matrix (ECM) scaffolds have been promising in various tissue engineering applications, such as the porcine small intestinal submucosa. Yet no particular scaffolds have been optimal for vocal fold regeneration. The decellularized human umbilical vein (HUV) is a novel allogeneic scaffold that has shown some promise for cardiovascular tissue engineering...
We investigate the anticancer effect of a traditional Chinese medicine gambogic acid (GA) in human gastric cancer line SGC-7901 and further study the mechanism of apoptosis induction of GA.Low differential human gastric cancer line SGC-7901 were treated with GA at different doses and different times, the inhibitory rates were detected by MTT assay. Apoptosis induced by GA in SGC-7901 cells was observed...
Objective: To construct the recombinant eukaryotic expression vector pcDNA3.1-CEMP1 containing the Human Cementum Protein 1 (hrCEMP1) by PCR and T/A cloning and establish NIH3T3 fibroblast cellline that stably expressing it. Methods: A pair of primers specific for amplifying the DNA fragment encoding hrCEMP1 were designed and synthesized. hrCEMP1 was inserted to the proper sites of vector pcDNA3.1...
Tissue engineered skin constructed by cultured seed cells provides a new way to accelerate skin wound healing and improve the quality of tissue repair. Conventional models with dermal scaffold material based on collagen hydrogels, however, have poor stability and unsatisfactory longevity. Here, we describe an improved acellular amniotic membrane as a scafford with epidermal stem cells for tissue engineering...
Background: CD44v6 is a splice variant of CD44 (CD44v) could probably promote cancer cells to adhere to vascular endothelium and base membranes and enhancing the invasion and metastasis of colonic carcinomas. Heat shock protein 72 (HSP72) as a molecular chaperone has been confirmed to overexpress in epithelial carcinoma cells. There may be a possible correlation between the expression of HSP72 and...
Endocrine disrupters (EDCs) have caused great concerns for the safety of water environment and drinking water in recent years. This paper uses lab-cultured human breast cancer cells (MCF-7) to examine the changes of water estrogenicity during the drinking water treatment process and uses chemical analysis as supplementary information. The results showed that MCF-7 were extremely sensitive to the aqueous...
MicroRNAs (miRNAs) were recently identified to play crucial roles in tumorigenesis processes. In present study, we explored the potential influence of miRNA-143 on several cellular biological processes related with lung cancer development in human bronchial epithelial cells 16-HBE. Chemosynthesis anti-miR-143 was used to down-regulate expression of miR-143. Cell proliferation was detected by methyl...
Cystathionine β-synthase deficiency is an inborn error of metabolism that is biochemically characterized by severe hyperhomocysteinemia and homocystinuria. Numerous cystathionine β-synthase mutants have so far been found in patients with cystathionine β-synthase deficiency. In this study, we use the cross-impact analysis to model the cystathionine βsynthase mutants with their clinical endpoints, and...
The analysis on codon usage bias of Us4 gene of Duck Plague Virus (DPV) may provide a basis for understanding the evolution and pathogenesis of DPV and for selecting appropriate host expression systems to improve the expression of target genes in vivo and in vitro. In this study, the synonymous codon usage in the Us4 gene of DPV and 17 reference herpesviruses have been investigated . The results reveals...
The G-banding technique is routinely used for generating characteristic banding patterns for chromosome identification and karyotyping. Since G-band patterns are not static, training cytogenetics technologists to master the skills of chromosome analysis is a long process. Furthermore, the opportunities for biology students to access a wide range of different chromosome aberrations for practice are...
Mitochondria are a vital organelle of eukaryotic cells. Many diseases are caused by the mitochondrial gene mutation , therefore, there is an urgent requirement to explore the proteins of mitochondrion. In this paper we have created a list of 1086 human mitochondrial genes by combining 13 datasets from publications or genomic experiments. Then we created a functional linkage network among mitochondrial...
We explore whether antisense oligodeoxy-nucleotides (ASODN) targeting survivin gene can reverse apoptotic resistance induced by cisplatin in human lung adenocarcinoma cells. A549 / CDDP cell lines were cultured routinely with RPMI-1640 medium. Survivin ASODN mediated by cytofectin was transfected into the A549 / CDDP cells. The influence of ASODN transfection on apoptosis was determined by Diphenylamine...
Photosensitization of quantum dots was activated with a LED light source (410nm) delivered at 5mw/cm2 to give a different dose by altering the radiation period. Cellular proliferative activity and apoptosis was measured using the cell counting kit-8 (CCK-8) assay. After four hours following photodynamic treatment, the degree of apoptosis was significantly increased, and the best experimental parameters...
Nitroreductase gene NOR1 is possibly involved in the chemical carcinogenesis of hepatic cancer and nasopharyngeal carcinoma. We have demonstrted that NOR1 overexpression could convert monofunction alalkylating agent, CB1954 ([5-aziridin-1-yl]-2,4-dinitrobenzamide) into a toxic form by reduction of the 4-nitro group of CB1954 and enhancing cell killing in nasopharyngeal carcinoma(NPC) cell line CNE...
Alpha-fetoprotein (AFP) is a major serum protein (Mr, =70,000) synthesized during fetal life. Reappearance of AFP in adult serum often signals pathological conditions, particularly hepatocarcinomas and teratocarcinomas. To obtain monoclonal antibodies against AFP for further study of the structure and biological function of AFP, a novel method for making hybridoma cell lines producing monoclonal antibodies...
Increasing reach on natural antioxidants in foods and development of new assays have prompted critical reflection on the field. There are lots of plants have antioxidant activity in natural. Anthocyanins are phenolic compounds widely distributed in fruits and vegetables and their consumption has been shown to prevent some chronic diseases. They possess antioxidant activities and could be involved...
Due to involvement in changing of chromatin structure, histone modifications are very important in regulation for gene expression. Some attempts have been done to resolve the relationship among histone modifications and gene expression. In this study, four types of Bayeasian network methods, Maximum weight spanning tree (MWST), K2, Markov Chain Monte Carlo (MCMC) and greedy-search(GS), are used to...
Three recombinant Escherichia coli strains were constructed to produce guanosine 5'-diphosphate (GDP)-mannose, donor of GDP-fucose, which is an essential substrate for synthesis of fucosyloligosaccharides. Glucokinase (glk), phosphomannomutase (manB), and mannose-1-phosphate guanylytransferase (manC), are three crucial enzymes for the de novo GDP-mannose biosynthesis, were overexpressed in recombinant...
Reactive oxygen species (ROS) and activation of redoxsensitive transcription factors such as nuclear factor (NF)-κB have been implicated in pro-inflammatory responses induced by nanoparticles. However, there is still only little knowledge about the pro-inflammatory responses induced by multi-walled carbon nanotubes (MWCNTs). The objective of the present study was to compare the ability of MWCNTs and...
Human Coagulation Factor IX (hFIX) plays a crucial role in the process of human endogenous blood clotting. Patients with deficiency of hFIX will suffer from hemophilia B. In this study, we use Pichia Pastoris to produce recombinant hFIX to cure hemophilia B Human liver hFIX cDNA were amplified by RT-PCR and inserted into pPIC9k vector, to construct pPIC9k-hFIX. The pPIC9k-hFIX were integrated into...
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