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Ancestry informative markers (AIMs) have been proven to contain necessary information for population classification. In this article, round robin symmetrical uncertainty ranking for preliminary AIM screening is proposed. Each single nucleotide polymorphism (SNP) is assigned a rank based on its ability to separate two populations from each other. In a multi-population scenario, all possible population...
To explore the inhibition of juglone in Qinglongyi on A-549 cells in vitro, MTT assay was used. Laser confocal scanning microscope was used to observe apoptotic morphology. Changes of cell cycle are studied by flow cytometry analysis. The result of MTT assay showed that juglone had a marked growth inhibition in A-549 cells and the IC50 is respectively 3.4 × 10-5 mol/L, 1.8 × 10-5 mol/L and 2.6 × 10...
To study the effect of Saponins of asparagus on HepG2 apoptosis, reactive oxygen species (ROS) and mitochondrial membrane potential (Δψm) levels. Saponins of asparagus with different concentration was treated with HepG2 at different time, MTT assay was used to detect inhibitory rate, fluorescence staining was used to observe apoptosis morphology, flow cytometry was used to detect apoptosis rate and...
Prion protein (PrP) is probably the only known infectious protein that possesses chameleon-like features, as reflected not only in its conformation but also in its function. Cellular PrP (PrPC), rich in α-helical structures, is believed to be functionally involved in neurotransmitter metabolism, immune cell activation, cell adhesion, signal transduction, copper metabolism, antioxidant activity, and...
Environmental risk is ascribed to contamination level as well as the potential decontamination capability of the contaminated sites. In some cases, natural attenuation by biodegradation may dominate self cleanup as microorganisms always try to survive, which reduces risk to human health. Conventional assessment is based on sequential monitoring of concentrations, which requires a large number of samples...
Cytochrome P450 4F3 (CYP4F3) catalyzes the inactivation of leukotriene B4 by ω-oxidation in human neutrophils. Here, we examined the mRNA and protein expression of the CYP4F3 in HL-60 cells, an acute promyelocytic leukemia cell line, in the presence of hydroquinone(HQ). We found hydroquinone led to a decrease in cell proliferation and a increase in cell apoptosis in a dose-dependent and time-dependent...
Codon usage preference and the highly expressed genes have strong correlations occur in many organisms. Codon usage preference of viruses may evolve much similar with its infected host to increase the fitness. In this study we investigated differences in codon usage preferences among influenza A H1N1 viruses which infected avian, swine and human, and may cause major pandemic around world. The relative...
Objective: Nonylphenol(NP) is NPnEOs of recalcitrant compound and classified as an endocrine disrupting chemical(EDC). The reproductive toxicity of Nonylphenol has been extensively studied in mammals. In this study, To explore the toxic effects of nonylphenol on human embryo Liver L-02 Cells. Methods Human embryo Liver L-02 Cells were exposed to different concentration of NP(1, 10, 50, 100μmol/L)...
For molecular biological detection of Mycobacterium tuberculosis, PCR methods with primers targeting different regions specific for mycobacterium tuberculosis complex are used worldwide. However, some uncertainties exist concerning the specificity of certain target regions and the sensitivity. To identify the methods which are best suited for diagnostics, 9 single-round and 2 multiple PCR systems...
MicroRNAs (miRNAs) are small noncoding RNAs which regulate gene expression. In this research, we used the Multiz alignments data of 44 Vertebrates to retrieve syntenic homolog sequences. And we identified 37 members of miR-1302 family among 71 potential homolog sequences. Then we analyzed the 37 homologs of hsa-mir-1302s by RepeatMasker and found all of these sequences are derived from MER53 elements...
We investigate the effect of specific antisense oligodeoxynucleotide (ASO DN) inhibition of Fas expression on T cell apoptosis induced by gastric carcinoma cell . Fas receptor (Fas) and Fas ligand (FasL) expressed by the gastric carcinoma cell line SGC-7901 and Jurkat T cells were detected by flow cytometry (FCM) and the ability of Fas L-inducing T cell apoptosis was tested by co-culture assay in...
Curcumin, the most active constituent of turmeric, is currently one of the major anticance new drugs and has been reported to enhance Heme Oxygenase-1(HO-1) in human brain when oxidative stress occur. Keeping the balance of HO-1 and HO-2 plays an important role in the brain. However, few researches study the effects of curcumin on the two isoenzymes of HO. Here we investigated the effects of curcumin...
Various phthalates are used extensively as plasticizers in kinds of consumer products. They are harmful for human reproduction and fertility. Mono-(2-ethylhexyl) phthalate (MEHP) is the active metabolite of di-(2-ethylhexyl) phthalate(DEHP),which is most commonly used as a plasticizer and solvent in numerous consumer products. MEHP has been demonstrated to be a reproductive toxicant in rodents, but...
Catechol-O-methyl transferase (COMT) influences dopamine concentration in the pre-frontal cortex (PFC). The G/A transition in the exon 4 of the COMT gene, which results in a valine (Val) to methionine (Met) amino acid substitution (Val158Met), can bring on different enzymatic activities. Much research has found that the Met/Met genotype associated with low enzymatic activity and may enhance cognitive...
Glycoprotein K encoded by the DEV-UL53 gene is one of the DEV envelope glycoproteins. Like most members of the alphaherpesvirinae, the genes in the UL region of its genome are well conserved, which means that the major DEV envelope glycoprotein K possessing similar functions of envelope glycoprotein K of alphaherpesvirinae plays an essential role in viral replication and cell fusion. Glycoprotein...
In order to explore the possibility of inhalation of formaldehyde on mouse bone marrow cells have an impact on cell cycle and use SPF level purely Kunming male mice for the study, with a concentration of 0.5 mg /m3, 1.0 mg/m3 and 3.0 mg/m3 of gaseous Formaldehyde dynamics in mice exposed to continuous 72 hours after the exposure using flow cytometry analysis cells from mouse bone marrow cells, cell...
This project focuses on the improvement of NDV production technology and innovation of the NDV vaccine preparation process with cell culture technology. Methods: To use primary chick embryo cell culture system in preparation of NDV anti-tumor vaccine. Results:In the new vaccine development process, the focused on maintaining and improving NDV titer while reducing the residual bovine serum. So that...
Endoplasmic reticulum stress(ERS)is a kind of sub-cellular pathological process which result from calcium disorderliness and protein improper folding. And endoplasmic reticulum stress induced apoptosis is a novel pathway which different from the mitochondria-initiated intrinsic pathway and the death receptor-triggered extrinsic pathway. Trichosanthin (TCS), a type I ribosome-inactivating protein,...
Adipose tissue is derived from the embryonic mesenchyme and can be isolated easily. The purpose of this study was to investigate the multipotent ability of adipose-derived mesenchymal stem cells and to investigate the molecular characterization of adipose-derived mesenchymal stem cells during chondrogenic differentiation in vitro. Our results showed that adipose-derived mesenchymal stem cells have...
Non-mitogenic form of recombinant Human acidic fibroblast growth factor (nrhaFGF) retained the cardio and neuro-protective characters of the wild-type aFGF while decreased the potential side effects like mitogenesis activity. In this paper, nrhaFGF was successfully expressed in Escherichia coli and yielded about 5% of the total soluble protein in the 10L fermentation tank. the succeedent chromatography,...
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