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Early perfusion is essential for survival and function of engineered tissues. A promising solution to this problem is the development of stable microvessels within an engineered construct. Transplantation of human endothelial cells within protein gels has been shown to form functional vascular networks both in vitro and after implantation into immunodeficient mice. Co-transplantation of human pericytes,...
Sepsis is the leading cause of death in critically ill patients in the United States. Current diagnosis of sepsis relies heavily on the patient's manifestation of septic symptoms, which occur at life-threatening late stage of sepsis. Because the underlying biological changes of sepsis occur hours to days before the clinical presentation of symptoms, early detection of the biological changes will provide...
Bone is a complex hierarchical natural composite. Its ability to accumulate damage and avert catastrophic failure is determined in part by its quality that is dependent on factors like bone material quality, bone micro-architecture and bone turnover. The hierarchy of bone, which ranges six orders of magnitude, also contributes significantly to toughening its matrix. Recently, non-collageneous proteins...
This work addresses the development of artificial cellular niches for studying the mechanisms that affect stem cell pluripotency, and aims at contributing to a better understanding of the cellular and molecular events that regulate stem cell functions. Embryonic stem (ES) cells were used as model systems to establish in vitro artificial microenvironments that better mimic the extracellular matrix...
In this paper, we construct a new mathematical model by incorporating both transcriptional/translational feedback loops and cAMP-mediated feedback loop into the mammalian circadian oscillator. Except for reproducing typical experimental observations, the model shows that (i) for a weak TTFL, cAMP can sustain transcriptional rhythms of the mammalian circadian clock, (ii) for a strong TTFL, cAMP can...
The structure and properties of scaffold are important in cell-based tissue engineering, especially the mechanical property. Here, we quantify the dynamic oscillatory mechanical behavior of two kinds of porous collagen/chitosan scaffolds. The Young's Modulus were measured in PBS using Atomic Force Microscopy (AFM)-based nano-indentation in response to an imposed oscillatory deformation as a function...
The aim of this study is to make a functional selection of the highly migratory orbital fat-derived stem cells (OFSCs) using a 3D collagen gel as a selection barrier. Firstly we isolated OFSCs by regular digestion. Passage 2-3 OFSCs were used to verify their multiple differentiation potentials. Then we encapsulated passage 2-6 OFSCs into collagen type I microspheres, followed by a well-established...
In extraction of information from the biomedical literature, name disambiguation of domain-specific entities, such as proteins, is one of the most important issues. The entity ambiguity with the highest dimension is the species to which an entity is associated with. Furthermore, one of the bottlenecks in inter-species gene name normalization is species disambiguation. To enhance the performance of...
Parkinson disease (PD) is well known that a neurodegenerative disorder characterized by selective loss of dopaminergic neurons in the substantia nigra pars compact (SN). Although the exact mechanism remains unclear, oxidative stress plays critical roles in the pathogenesis of PD. DJ-1 is a multifunctional protein, a potent antioxidant and chaperone, linked to autosomal recessive early onset of PD...
Metallothioneins (MTs) are small cysteine-rich proteins that are involved in many diverse biological processes, which are interesting especially due to their influence on therapy of cancer. The primary structures of MTs from different organisms are various despite they have the same purpose - a transport of metal ions in organisms. Every single mutation in amino acid sequences can have a drastic effect...
One of the most important clinical problems in caring for elderly patients is treatment of pressure ulcers. One component of normal wound healing is the generation of an inflammatory reaction, which is characterized by the sequential infiltration of neutrophils, macrophages and lymphocytes. In aged C57BL/6 mice, wound healing is relatively inefficient. We examined the effects of monocytes lineage...
With the increased availability of genome-scale data, it becomes possible to study functional relationships of genes across multiple biological networks. While most previous approaches for studying conservation of patterns in networks are through the application of network alignment algorithms or the identification of network motifs, we show that it is possible to exhaustively enumerate all graphlet...
Ginger (Zingiber officinale) is used as a cooking spice in the diet in many parts of the world. Recent research has found ginger to have various pharmacological properties due to a variety of active constituents, including shogaols and gingerols. The aim of the present study was to assess the effect of alcohol extract of Zingiber officinale on the fractions of serum protein in mice. For this purpose,...
This paper proposes a novel method for analyzing similarities of protein sequences based on features generated from the hydropathy properties of amino acid sequences. At first using the hydropathy characteristics, the protein sequences are converted into fixed-dimensional feature vectors. Based on these feature vectors, we analyze nine ND5 proteins and construct a phylogenic tree as an application...
The infrared beamline at MAX-lab in Lund is equipped with a Bruker Hyperion 3000 microscope combined with an IFS/66v FTIR instrument. In combination with a number of different detectors and objectives this makes it possible to study a large number of different samples. For example there is a FPA detector for mapping larger areas and a Bolometer for measurements in the far infrared. A number of examples...
Prion protein (PrP) is probably the only known infectious protein that possesses chameleon-like features, as reflected not only in its conformation but also in its function. Cellular PrP (PrPC), rich in α-helical structures, is believed to be functionally involved in neurotransmitter metabolism, immune cell activation, cell adhesion, signal transduction, copper metabolism, antioxidant activity, and...
White noise makes peroxidative damage of the brain, dose it have effect on the phosphorylation of tau in hippocampus? We used 66 mice exposed to 80 dB SPL white noise for 3 days, 1 week, and 3 weeks respectively, then detected the changes of Superoxide dismulase (SOD) activity and malondialdehyde (MDA) content in inferior colliculus (IC), auditory cortex (AC), and hippocampus. Meanwhile we detected...
We studied the Fas/anti-Fas system to induce killing of Fas-expressing immunocytes with resultant immunosuppression. W7TM-1 tumour cells, a rat T-cell line, were inoculated subcutaneously in BALB/c mice and tumour growth was monitored in untreated mice and in mice treated with RMF2. Prior to treatment with RMF2, we examined the expression of Fas in isolated splenocytes and in tumour infiltrating lymphocytes...
To explore oxidative damage induced by PBDE 209 and its mechanism we carried out this research. Mice were given intragastrically with PBDE 209 via a metal gastric-tube at different levels (0, 50, 100, 200 mg/(kg·d)) for seven days and the malondialdehyde (MDA) content, superoxide dismutase (SOD) activities in liver and liver index were mensurated. Results showed that along with elevation of PBDE 209...
In this study, artificial antigens for Bensulfuron-methyl (BSM) were prepared by means of two-step procedure of glutaraldehyde method. The hapten BSM was covalently attached to ovalbumin to synthesis immunogen (BSM-OVA), and hapten Benzyl sulfonamide (BS) was linked to BSA to prepare coating antigen (BS-BSA). The conjugates were identified by UV spectrophotometry and SDS-PAGE. After immunized with...
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