SobrinoCorresponding author. Tel.: +34 1 6202300; fax: +34 1 6202247; e-mail: sobrino@samba.cnb.uam.esTecnologı́a para Diagnóstico e Investigación S.A., Alcobendas 28100, Madrid, SpainCentro de Investigación en Sanidad Animal, INIA, Valdeolmos 28130, Madrid, SpainCentro de Biologı́a Molecular `Severo Ochoa', Cantoblanco 28049, Madrid, SpainA RT-PCR assay based on specific amplification of RNA sequences from each of the etiological agents of three important vesicular diseases that affect swine, foot-and-mouth disease virus (FMDV), swine vesicular disease virus (SVDV), and vesicular stomatitis virus (VSV), was developed. Genotype-specific primers that amplified DNA fragments of differential size from SVDV 3D gene or VSV L gene were selected with the aid of a computer program. Experimental testing of the primers predicted as SVDV-specific identified a primer pair, SA2/SS4, that rendered a specific product from SVDV RNAs, but did not amplify RNA from either FMDV or coxsackie B5 virus (CV-B5), a highly related picornavirus. Primers SA2/SS4 were used in combination with primers 3D2/3D1, which amplify a product of different size on FMDV 3D gene (ı́
A RT-PCR assay based on specific amplification of RNA sequences from each of the etiological agents of three important vesicular diseases that affect swine, foot-and-mouth disease virus (FMDV), swine vesicular disease virus (SVDV), and vesicular stomatitis virus (VSV), was developed. Genotype-specific primers that amplified DNA fragments of differential size from SVDV 3D gene or VSV L gene were selected...